Sun D, Abraham S N, Beachey E H
University of Tennessee, Memphis.
Antimicrob Agents Chemother. 1988 Aug;32(8):1274-7. doi: 10.1128/AAC.32.8.1274.
We investigated the influence of berberine sulfate, an ancient Chinese antibiotic, upon the adhesion of uropathogenic Escherichia coli to erythrocytes and epithelial cells. Although berberine sulfate in increasing concentrations had no effect on bacterial growth or on the synthesis of major outer membrane proteins of the E. coli organisms, it increasingly blocked adhesion. The decreased adhesion was accompanied by a reduction in the synthesis of fimbrial subunits and in the expression of assembled fimbriae. These results suggest that the anti-infectious activity of berberine sulfate in E. coli-induced urinary tract infections may be mediated by the selective suppression of the synthesis and assembly of fimbriae by uropathogenic organisms.
我们研究了中国古代抗生素硫酸小檗碱对尿路致病性大肠杆菌黏附红细胞和上皮细胞的影响。尽管浓度不断增加的硫酸小檗碱对细菌生长或大肠杆菌主要外膜蛋白的合成没有影响，但它越来越多地阻断黏附。黏附减少伴随着菌毛亚基合成减少和组装菌毛表达减少。这些结果表明，硫酸小檗碱在大肠杆菌引起的尿路感染中的抗感染活性可能是通过选择性抑制尿路致病性微生物菌毛的合成和组装来介导的。
