Moriya Takeo, Satomi Yoshinori, Kobayashi Hiroyuki
Integrated Technology Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited, 26-1 Muraoka-Higashi 2-chome, Fujisawa, Kanagawa 251-8555, Japan.
J Biochem. 2018 Feb 1;163(2):113-121. doi: 10.1093/jb/mvx066.
While endogenous metabolites in plasma can be used as clinical biomarkers, intra-day variations should be carefully considered. The postprandial effect is a large contributing factor and is dependent on regional features (e.g. meals, ethnicity). Thus, for clinical application, regional-specific postprandial baseline data are required. In this study, 10 healthy Japanese volunteers of different ages and genders ate the same meal, and blood samples were taken 30 min before and 1 h after the meal challenge. Plasma metabolomics was conducted and metabolites that significantly changed with the meal challenge were extracted. Principal component analysis of the data from 1101 metabolites showed a postprandial shift with a common direction despite marked individual variation. Pathway enrichment analysis demonstrated known postprandial effects, including the energy utilization shift from lipolysis to glycolysis and the elevation of bile acids for lipid absorption. Other postprandial metabolic changes were observed, including decreases in orexigenic signals and increases of food-derived components. The postprandial alteration accumulated in this study will be used for the understanding of Japanese clinical metabolomics for health promotion in Japan.
虽然血浆中的内源性代谢物可作为临床生物标志物，但应仔细考虑日内变化。餐后效应是一个重要因素，并且取决于区域特征（例如饮食、种族）。因此，对于临床应用，需要区域特异性的餐后基线数据。在本研究中，10名不同年龄和性别的健康日本志愿者食用相同的餐食，并在餐后挑战前30分钟和餐后1小时采集血样。进行了血浆代谢组学分析，并提取了随餐后挑战而显著变化的代谢物。对1101种代谢物的数据进行主成分分析表明，尽管个体差异明显，但餐后变化具有共同的方向。通路富集分析显示了已知的餐后效应，包括能量利用从脂肪分解向糖酵解的转变以及胆汁酸升高以促进脂质吸收。还观察到其他餐后代谢变化，包括促食欲信号的降低和食物衍生成分的增加。本研究中积累的餐后变化将用于理解日本临床代谢组学，以促进日本的健康。
