Jin Jian, Zhong Can, Qin You, Cai Yuan, Zhen Lanping, Shen Bingbing, Chen Lin, Wan Dan, Qin Yuhui, Zhang Shuihan
Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, PR China.
Hunan University of Chinese Medicine, Changsha 410208, PR China.
FEMS Microbiol Lett. 2017 Nov 1;364(20). doi: 10.1093/femsle/fnx181.
Caterpillar fungi have numerous pharmacological and therapeutic applications in traditional medicine, due to a variety of active chemical constituents, such as cordycepin and adenosine. It is imperative to discover new resource for artificial cultivation and biometabolite production since the traditional natural species are endangered. In this study, a new strain HACM 001 was isolated and identified as Ophiocordyceps xuefengensis by rDNA-ITS sequencing. This strain showed the potential of artificial infection to caterpillar larvae leading to mummification, as well as fermentation mycelia in liquid culture and cultivation stromata in solid medium. Eight nucleosides and nucleobases, especially cordycepin and adenosine, were determined and analyzed with HPLC-DAD-Q-TOF-MS/MS technology. Cordycepin was detected in all forms of present O. xuefengensis strain at different contents, among which the highest content (37.1 μg/g) appeared in the stromata cultivated on solid medium. The content of adenosine in mycelia and stromata, respectively, reached 1155 μg/g and 1470 μg/g. Therefore, O. xuefengensis might be an alternative source for obtaining artificial fungus-caterpillar-larvae complex and producing cordycepin and adenosine.
由于含有多种活性化学成分，如虫草素和腺苷，冬虫夏草在传统医学中有众多药理和治疗应用。鉴于传统的天然品种濒临灭绝，因此有必要寻找新的人工栽培资源和生物代谢产物生产资源。在本研究中，通过rDNA-ITS测序分离并鉴定出一种新菌株HACM 001为雪峰虫草。该菌株显示出人工感染毛虫幼虫导致木乃伊化的潜力，以及在液体培养中产生发酵菌丝体和在固体培养基中产生栽培子座的潜力。采用HPLC-DAD-Q-TOF-MS/MS技术测定并分析了8种核苷和碱基，特别是虫草素和腺苷。在所有形式的现有雪峰虫草菌株中均检测到了不同含量的虫草素，其中在固体培养基上培养的子座中含量最高（37.1μg/g）。菌丝体和子座中腺苷的含量分别达到1155μg/g和1470μg/g。因此，雪峰虫草可能是获取人工虫草复合体以及生产虫草素和腺苷的替代资源。
