Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China.
Food Funct. 2017 Nov 15;8(11):4251-4264. doi: 10.1039/c7fo00880e.
Panax notoginseng medicinal liquor (PML) has a long history of use in the function of blood circulation. However, the processing of PML is currently dependent on experience, which results in low efficiency and unstable quality of PML. A variety of substances of P. notoginseng have a strong ability to scavenge free radicals and antioxidant activity, but the antioxidant activity of PML has not been formally researched. The aim of the present study was to optimize the processing technology of PML and to verify the anti-oxidation and anti-deposition of melanin functions of PML. Based on the Box-Behnken design of response surface method, the PML processing parameters were established as follows: the ratio of liquid to solid 32 : 1, 53% of alcohol, and soaking time of 35 d. With elevating concentration of PML extract, the reducing force and scavenging rates of DPPH, superoxide anion, hydroxyl radical and ABTS were increased, and the inhibition rate of tyrosinase activity and the melanin synthesis ability were increased in mice melanoma B16 cells. Thus, the optimal processing technology not only shortened the processing time but also decreased the material costs. PML may be developed as food or beauty products for the functions of anti-oxidation and anti-deposition of melanin.
三七药酒（PML）在活血化瘀方面有着悠久的应用历史。然而，目前 PML 的加工仍依赖于经验，导致 PML 的效率低下且质量不稳定。多种三七成分具有较强的自由基清除能力和抗氧化活性，但 PML 的抗氧化活性尚未得到正式研究。本研究旨在优化 PML 的加工工艺，并验证 PML 的抗黑色素沉积和抗氧化功能。基于响应面法的 Box-Behnken 设计，确定 PML 的加工参数如下：液固比 32：1、酒精浓度 53%、浸泡时间 35 d。随着 PML 提取物浓度的升高，还原力和 DPPH、超氧阴离子、羟自由基和 ABTS 的清除率增加，对酪氨酸酶活性的抑制率和黑色素合成能力在小鼠黑色素瘤 B16 细胞中也增加。因此，优化的加工工艺不仅缩短了加工时间，还降低了材料成本。PML 可能被开发为具有抗氧化和抗黑色素沉积功能的食品或美容产品。
