School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Molecules. 2018 Oct 10;23(10):2586. doi: 10.3390/molecules23102586.
(1) Background: Application of echinacoside has become increasingly important for its significant biological activities. However, there are many disadvantages in existing synthesis methods such as contaminating the environment, harsh reaction conditions and so on. Therefore, it is urgent to invent a novel alternative method that can increase the yield of echinacoside. (2) Methods: In this study, we isolated and purified an endophyte from the leaves of Ait. Then, we improved the yield of echinacoside by optimizing the fermentation condition with an endophytic fungus. sp. H1 was isolated from Ait. In addition, response surface methodology was used to optimize the fermentation condition. (3) Results: The results indicate that the maximal yield of echinacoside (37.16 mg/L) was obtained when inoculation rate, temperature and days were 13.98%, 27.85 °C and 26.06 days, respectively. The yield of echinacoside was 150.47 times higher under the optimal conditions than under the control conditions. The results indicate that the yield of echinacoside could be improved with endophytic fermentation by optimizing the fermentation condition. We provide an alternative method for echinacoside production by endophytic fermentation in this paper. It may have a profound effect on the application of echinacoside.
(1) 背景：由于其显著的生物活性，毛蕊花糖苷的应用变得越来越重要。然而，现有的合成方法存在许多缺点，如环境污染、苛刻的反应条件等。因此，急需发明一种能够提高毛蕊花糖苷产量的新型替代方法。
(2) 方法：本研究从 叶中分离和纯化了一种内生真菌。然后，我们通过优化内生真菌的发酵条件来提高毛蕊花糖苷的产量。 sp. H1 从 中分离得到。此外，还采用响应面法优化了发酵条件。
(3) 结果：结果表明，当接种率、温度和天数分别为 13.98%、27.85°C 和 26.06 天时，毛蕊花糖苷的最大产量（37.16mg/L）。在最佳条件下，毛蕊花糖苷的产量比对照条件下提高了 150.47 倍。结果表明，通过优化发酵条件，内生发酵可以提高毛蕊花糖苷的产量。本文通过内生发酵生产毛蕊花糖苷提供了一种替代方法，可能对毛蕊花糖苷的应用产生深远影响。
