Cousillas H, Cole K S, Johnstone B M
Department of Physiology, University of Western Australia, Nedlands.
Hear Res. 1988 Nov;36(2-3):213-20. doi: 10.1016/0378-5955(88)90063-9.
Venom from the spider Argiope trifasciata, a highly specific blocker of the ionic channels associated with invertebrate glutamatergic receptors, was perfused through scala tympani of the basal turn of the pig cochlea. Its effect on spontaneous and driven activity of single afferent neurons was studied. 0.1 U/ml spider venom altered the maximum driven activity without an effect on spontaneous activity. 1 U/ml spider venom suppressed both spontaneous and driven activity. These findings are consistent with the hypothesis that L-glutamate is the neurotransmitter of the synapse between inner hair cells and primary auditory afferent neurons. The results also suggest that the differences in spontaneous activity between neurons may originate in variations in neurotransmitter release.
来自三带金蛛的毒液是一种与无脊椎动物谷氨酸能受体相关的离子通道的高度特异性阻滞剂，它被灌注到猪耳蜗基底转的鼓阶中。研究了其对单个传入神经元自发活动和驱动活动的影响。0.1 U/ml的蜘蛛毒液改变了最大驱动活动，但对自发活动没有影响。1 U/ml的蜘蛛毒液抑制了自发活动和驱动活动。这些发现与L-谷氨酸是内毛细胞和初级听觉传入神经元之间突触的神经递质这一假设一致。结果还表明，神经元之间自发活动的差异可能源于神经递质释放的变化。
