Pujol R, Lenoir M, Robertson D, Eybalin M, Johnstone B M
Hear Res. 1985 May;18(2):145-51. doi: 10.1016/0378-5955(85)90006-1.
Cochleas of adult guinea pigs and rats, and 6-day-old rat pups, were injected, through the round window, with 2 microliters of artificial classical Konishi perilymph containing 1 nmol kainic acid (KA). 5 min later, they were fixed, removed, and processed for electron microscopy. In all KA-treated cochleas, the injection resulted in a severe swelling of auditory dendrites below the inner hair cells (IHCs). Below the outer hair cells (OHCs), the swelling appeared only in the 6-day-old rats, not in adult animals. These results are significant in three different ways: (1) They confirm the strong difference between afferents innervating the IHCs and the OHCs in adult cochleas. (2) They shed some light on the synaptic plasticity found at the OHC level during synaptogenesis. (3) They support the hypothesis that glutamate, or a related substance, is the IHC neurotransmitter.
通过圆窗向成年豚鼠、大鼠以及6日龄大鼠幼崽的耳蜗内注射2微升含有1纳摩尔 kainic 酸（KA）的人工经典小西外淋巴。5分钟后，将它们固定、取出并进行电子显微镜处理。在所有接受KA处理的耳蜗中，注射导致内毛细胞（IHC）下方的听觉树突严重肿胀。在外毛细胞（OHC）下方，肿胀仅出现在6日龄大鼠中，而在成年动物中未出现。这些结果在三个不同方面具有重要意义：（1）它们证实了成年耳蜗中支配IHC和OHC的传入神经之间存在显著差异。（2）它们为突触形成过程中OHC水平发现的突触可塑性提供了一些线索。（3）它们支持谷氨酸或相关物质是IHC神经递质的假说。
