State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.
Bioresour Technol. 2021 Oct;337:125506. doi: 10.1016/j.biortech.2021.125506. Epub 2021 Jul 6.
As a key anti-degradation barrier that restricts the biotransformation of lignocellulose, the presence of lignin usually severely affects the quality of the extracted xylo-oligosaccharides (XOS). Herein, this study proposed a practical route to improve the quality and antioxidant activity of XOS extracted from lignocellulosic biomass via selective removal of lignin. The highest delignification of 92.6% was successfully achieved with 8% sodium chlorite at 75°C for 2 h. An ideal hemicellulose sample with a purity of 86.1% was obtained by selective removal of lignin. A high-quality XOS sample with a purity of 96.3%, a yield of 77.4%, and a color value of 814 was obtained by separating and purifying the enzymatic hydrolysate. Antioxidant activity assay showed that the highest radical scavenging activity of XOS was 87.3%. Importantly, this study provide a feasible and effective route for the lignocellulosic biomass utilization strategy based on the selective removal of lignin.
木质素作为限制木质纤维素生物转化的关键降解屏障，其存在通常严重影响提取的木寡糖（XOS）的质量。在此，本研究提出了一种实用的路线，通过选择性去除木质素来提高从木质纤维素生物质中提取的 XOS 的质量和抗氧化活性。在 75°C 下用 8%次氯酸钠处理 2 小时，成功实现了高达 92.6%的最高脱木质素率。通过选择性去除木质素，获得了纯度为 86.1%的理想半纤维素样品。通过分离和纯化酶解产物，获得了纯度为 96.3%、得率为 77.4%、色值为 814 的高质量 XOS 样品。抗氧化活性测定表明，XOS 的最高自由基清除活性为 87.3%。重要的是，该研究为基于选择性去除木质素的木质纤维素生物质利用策略提供了一种可行且有效的途径。
