Department of Respiratory Medicine, Jining NO.1 People's Hospital, Jining, Shandong Province, China.
Sci Rep. 2017 Oct 23;7(1):13807. doi: 10.1038/s41598-017-13956-2.
Heat shock protein 27 (HSP27) has been found to be related to tumorigenesis. The aim of this study was to investigate the expression pattern and clinical significance of HSP27 in non-small-cell lung cancer (NSCLC). The expression of HSP27 in tissues was examined by immunohistochemistry and serum level of HSP27 mRNA was detected by real-time PCR. The survival analysis was performed by a Kaplan Meier method and the estimation of risk factors was determined by the multiple regression analysis. The expression of HSP27 was increased in lung cancer tissues (p < 0.001) and serum (p < 0.001) of NSCLC patients and higher HSP27 in lung cancer tissues and serum of NSCLC patients was associated with poorly differentiated cancer (p < 0.001; p = 0.035), lymphatic metastasis (p < 0.001; p < 0.001), advanced TNM stage (p < 0.001; p < 0.001). And the levels of HSP27 in tissues and serum of lung cancer patients had a certain positive correlation (p = 0.046). Moreover, increased HSP27 expression correlated with shorter survival of NSCLC patients (p < 0.001). The results suggest that HSP27 may serve as a potential biomarker for diagnosis and prognosis of NSCLC.
热休克蛋白 27（HSP27）已被发现与肿瘤发生有关。本研究旨在探讨 HSP27 在非小细胞肺癌（NSCLC）中的表达模式及其临床意义。采用免疫组织化学法检测 HSP27 在组织中的表达，采用实时 PCR 法检测 HSP27 mRNA 在血清中的水平。采用 Kaplan-Meier 法进行生存分析，采用多因素回归分析确定危险因素的估计。HSP27 在肺癌组织（p<0.001）和非小细胞肺癌患者血清（p<0.001）中的表达增加，HSP27 在肺癌组织和血清中的表达水平与癌症分化不良（p<0.001;p=0.035）、淋巴转移（p<0.001;p<0.001）、晚期 TNM 分期（p<0.001;p<0.001）有关。而且，肺癌患者组织和血清中 HSP27 的水平呈正相关（p=0.046）。此外，HSP27 表达增加与 NSCLC 患者的生存时间缩短相关（p<0.001）。结果表明，HSP27 可能作为 NSCLC 诊断和预后的潜在生物标志物。
