人嗅黏膜间充质干细胞向光感受器细胞的分化
Differentiation of human olfactory mucosa mesenchymal stem cells into photoreceptor cells .
作者信息
Lu Wen, Duan Da, Ackbarkhan Zacharia, Lu Ming, Huang Min-Li
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
Department of Neurosurgery, the 163rd Hospital of Chinese PLA, the Second Affiliated Hospital of Hunan Normal University, Changsha 410003, Hunan Province, China.
出版信息
Int J Ophthalmol. 2017 Oct 18;10(10):1504-1509. doi: 10.18240/ijo.2017.10.04. eCollection 2017.
AIM
To investigate whether the human olfactory mucosa mesenchymal stem cells (OM-MSCs) can differentiate into photoreceptor cells .
METHODS
Through the olfactory mucosa adherent method, olfactory mucosa was isolated, cultured and identified among mesenchymal stem cells. The cell surface markers were analyzed by flow cytometry, induced to differentiate into retinal photoreceptor cells , and the expression of rhodopsin was observed and identified by Immunofluorescence and Western blot methods.
RESULTS
OM-MSCs from human were spindle cell-based, and showing radial colony arrangement. OM-MSCs were negative for CD34, CD45 and CD105, but positive for CD73 and CD90. Following induction, a strong positive reaction was produced by photoreceptor specific marker rhodopsin in the cells.
CONSLUSION
This novel finding demonstrates that OM-MSCs can be cultured and expanded . They possess biological characteristics of mesenchymal stem cells, and have the ability to be induced into retinal cells.
目的
研究人嗅黏膜间充质干细胞（OM-MSCs）是否能分化为光感受器细胞。
方法
通过嗅黏膜贴壁法分离、培养并鉴定嗅黏膜间充质干细胞。采用流式细胞术分析细胞表面标志物，诱导其分化为视网膜光感受器细胞，并用免疫荧光和蛋白质印迹法观察和鉴定视紫红质的表达。
结果
人OM-MSCs以梭形细胞为主，呈放射状集落排列。OM-MSCs对CD34、CD45和CD105呈阴性，但对CD73和CD90呈阳性。诱导后，细胞中光感受器特异性标志物视紫红质产生强阳性反应。
结论
这一新颖发现表明OM-MSCs可培养和扩增。它们具有间充质干细胞的生物学特性，并具有被诱导分化为视网膜细胞的能力。
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