Centre for Clinical Oral Research, Institute of Dentistry, Queen Mary University of London (QMUL), Barts and The London School of Medicine and Dentistry, London, UK.
Centre for Oral Immunobiology and Regenerative Medicine, Queen Mary University of London (QMUL), Bart's & The London School of Dentistry & Medicine, London, UK.
J Periodontal Res. 2018 Apr;53(2):174-187. doi: 10.1111/jre.12498. Epub 2017 Oct 24.
There is significant evidence that, during the early stages of osseointegration, moderately rough hydrophilic (SLActive) surfaces can accelerate osteogenesis and increase bone-to-implant contact in comparison to hydrophobic (SLA) surfaces. However, very little is known regarding the molecular mechanisms behind the influence that surface chemistry modifications to increase hydrophilicity determine on bone healing. The aim of this study was to describe for the first time the proteins and related signalling pathways expressed during early osseous healing stages under SLA and SLActive titanium domes for guided bone regeneration.
One SLA and 1 SLActive dome with an internal diameter of 5.0 mm and a height of 3.0 mm were secured to the parietal bones of nine 6-month-old male New Zealand rabbits. Three animals were randomly euthanized at 4, 7 and 14 days and the newly formed tissues retrieved under the domes were analysed with liquid chromatography-mass spectrometry/mass spectrometry. STRING and KEGG databases were applied for Gene Ontology and pathway analyses.
A different modulation of several pathways was detected between the 2 groups at all healing times. The main differences in the osseous healing response associated to the 2 surfaces were related to pathways involved in regulating the inflammatory response, differentiation of osteoblast precursors and skeletogenesis. At day 7, the highest number of proteins and the highest cellular activity were observed in both groups, although a more complex and articulated proteome in terms of cellular metabolism and signal transduction was observed in SLActive samples.
This is the first study describing the proteome expressed during early healing stages of guided bone regeneration and osseointegration. A combination of enhanced early osteogenic response and reduced inflammatory response were suggested for the hydrophilic group. Future studies are needed to corroborate these findings and explore the molecular effects of different titanium surfaces on the cascade of events taking place during bone formation.
有大量证据表明，在骨整合的早期阶段，与疏水性（SLA）表面相比，中等粗糙度的亲水性（SLActive）表面可以加速成骨并增加骨与种植体的接触。然而，对于表面化学修饰增加亲水性对骨愈合的影响背后的分子机制知之甚少。本研究的目的是首次描述在 SLA 和 SLActive 钛穹顶引导骨再生的早期骨愈合阶段中表达的蛋白质和相关信号通路。
将一个直径为 5.0mm、高度为 3.0mm 的 SLA 和一个 SLActive 穹顶固定在 9 只 6 月龄雄性新西兰兔的顶骨上。随机选择 3 只动物在 4、7 和 14 天处死，在穹顶下取出新形成的组织，用液相色谱-质谱/质谱进行分析。STRING 和 KEGG 数据库用于基因本体论和途径分析。
在所有愈合时间，两组之间检测到几个途径的不同调节。与两种表面相关的骨愈合反应的主要差异与调节炎症反应、成骨前体细胞分化和骨骼发生的途径有关。在第 7 天，两组都观察到最多的蛋白质和最高的细胞活性，但在 SLActive 样本中观察到更复杂和复杂的细胞代谢和信号转导蛋白组。
这是首次描述引导骨再生和骨整合早期愈合阶段表达的蛋白质组学。亲水组被认为具有增强的早期成骨反应和减轻炎症反应的组合。需要进一步的研究来证实这些发现，并探索不同钛表面对骨形成过程中发生的事件级联的分子影响。
