Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway.
Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway.
Molecules. 2017 Oct 24;22(10):1806. doi: 10.3390/molecules22101806.
A Western lifestyle with low physical activity and a diet rich in sugar, fat and processed food contribute to higher incidences of diabetes and obesity. Enhanced glucose uptake in human liver cells was observed after treatment with phenolic extracts from different Nordic berries. All berry extracts showed higher inhibition against α-amylase and α-glucosidase than the anti-diabetic agent acarbose. Total phenolic content and phenolic profiles in addition to antioxidant activities, were also investigated. The berries were extracted with 80% methanol on an accelerated solvent extraction system (ASE) and then purified by C-18 solid phase extraction (SPE). Among the ASE methanol extracts, black chokeberry, crowberry and elderberry extracts showed high stimulation of glucose uptake in HepG2 cells and also considerable inhibitory effect towards carbohydrate hydrolyzing enzymes. SPE extracts with higher concentrations of phenolics, resulted in increased glucose uptake and enhanced inhibition of α-amylase and α-glucosidase compared to the ASE extracts. Crowberry and cloudberry were the most potent 15-lipoxygenase inhibitors, while bog whortleberry and lingonberry were the most active xanthine oxidase inhibitors. These results increase the value of these berries as a component of a healthy Nordic diet and have a potential benefit against diabetes.
西方的生活方式通常运动量低，饮食中富含糖、脂肪和加工食品，这导致糖尿病和肥胖的发病率更高。用来自不同北欧浆果的酚类提取物处理后，观察到人肝细胞中的葡萄糖摄取增强。与糖尿病药物阿卡波糖相比，所有浆果提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用都更高。此外，还研究了总酚含量和酚类谱以及抗氧化活性。浆果在加速溶剂萃取系统 (ASE) 中用 80%甲醇提取，然后用 C-18 固相萃取 (SPE) 纯化。在 ASE 甲醇提取物中，黑果腺肋花楸、越桔和接骨木提取物对 HepG2 细胞中葡萄糖摄取的刺激作用最强，对碳水化合物水解酶的抑制作用也相当大。与 ASE 提取物相比，具有更高酚类浓度的 SPE 提取物可增加葡萄糖摄取并增强对α-淀粉酶和α-葡萄糖苷酶的抑制作用。越桔和云莓是最有效的 15-脂氧合酶抑制剂，而沼泽越桔和蔓越莓是最活跃的黄嘌呤氧化酶抑制剂。这些结果增加了这些浆果作为健康北欧饮食的一部分的价值，并具有预防糖尿病的潜在益处。
