Yuan Xiao-Long, Mao Xin-Xin, Liu Xin-Min, Cheng Sen, Zhang Peng, Zhang Zhong-Feng
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
Shanghai Tobacco Group Company Limited, Shanghai, China.
Genet Mol Biol. 2017 Oct-Dec;40(4):844-854. doi: 10.1590/1678-4685-GMB-2016-0308. Epub 2017 Oct 23.
Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-throughput sequencing platform. The circular mitogenome was found to be 28,081 nucleotides in length and comprised of 17 protein-coding genes, 24 tRNA genes, and 2 rRNA genes. The nucleotide composition of the genome was A+T-biased (74.13%). Group-II introns were found in the nad1, nad5, and cob genes. The most frequently used codon of protein-coding genes was UAU. Isoleucine was identified as the most common amino acid, while proline was the least common amino acid in protein-coding genes. The gene-arrangement order is nearly the same when compared with other Ascomycota mitogenomes. Phylogenetic relationships based on the shared protein-coding genes revealed that E. album is closely related to the Cordycipitaceae family, with a high-confidence support value (100%). The availability of the mitogenome of E. album will shed light on the molecular systematic and genetic differentiation of this species.
白色内齿菌是一种广泛传播的病原体，可在人类和动物中引起多种皮肤病和呼吸道疾病。尽管其具有危害性，但该物种的基本遗传和分子背景仍知之甚少。在本研究中，利用高通量测序平台测定了白色内齿菌的线粒体基因组序列。发现环状有丝分裂基因组长度为28,081个核苷酸，由17个蛋白质编码基因、24个tRNA基因和2个rRNA基因组成。基因组的核苷酸组成偏向A+T（74.13%）。在nad1、nad5和cob基因中发现了II组内含子。蛋白质编码基因最常用的密码子是UAU。异亮氨酸被确定为蛋白质编码基因中最常见的氨基酸，而脯氨酸是最不常见的氨基酸。与其他子囊菌有丝分裂基因组相比，基因排列顺序几乎相同。基于共享蛋白质编码基因的系统发育关系表明，白色内齿菌与虫草科密切相关，具有高置信度支持值（100%）。白色内齿菌有丝分裂基因组的可用性将为该物种的分子系统学和遗传分化提供线索。
