Shenzhen Key Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, China.
National Institutes of Science and Technology, Gaithersburg, MD, United States.
Curr Med Chem. 2019;26(21):4029-4041. doi: 10.2174/0929867324666171024101715.
Luminescence bioimaging is widely used for noninvasive monitoring of biological targets in real-time with high temporal and spatial resolution. For efficient bioimaging in vivo, it is essential to develop smart organic dye platforms. Fluorescein (FL), a traditional dye, has been widely used in the biological and clinical studies. However, visible excitation and emission limited their further application for in vivo bioimaging. Nearinfrared (NIR) dyes display advantages of bioimaging because of their minimum absorption and photo-damage to biological samples, as well as deep tissue penetration and low auto-luminescence from background in the living system. Thus, some great developments of near-infrared fluorescein-inspired dyes have emerged for bioapplication in vitro and in vivo. In this review, we highlight the advances in the development of the near-infrared chemodosimeters for detection and bioimaging based on the modification of fluoresceininspired dyes naphtho-fluorescein (NPF) and cyanine-fluorescein (Cy-FL).
荧光生物成像广泛用于实时、高时空分辨率的生物靶标非侵入性监测。为了实现高效的活体生物成像，开发智能有机染料平台至关重要。荧光素（FL）作为一种传统染料，已广泛应用于生物学和临床研究中。然而，其可见激发和发射限制了其在活体生物成像中的进一步应用。近红外（NIR）染料由于对生物样本的最小吸收和光损伤，以及在活体系统中更深的组织穿透和低背景自荧光，在生物成像中具有优势。因此，一些基于近红外荧光素启发的染料的新型近红外荧光染料已经出现，用于体外和体内的生物应用。在本文中，我们重点介绍了基于萘基荧光素（NPF）和花菁-荧光素（Cy-FL）染料修饰的近红外化学传感器在检测和生物成像方面的发展进展。
