Department of Chemistry & State Key Laboratory of Molecular Engineering of Polymers & Institute of Biomedicine Sciences & Collaborative Innovation Center of Chemistry for Energy Materials , Fudan University , 220 Handan Road , Shanghai 200433 , P. R. China.
ACS Appl Mater Interfaces. 2019 Jan 9;11(1):430-436. doi: 10.1021/acsami.8b19961. Epub 2018 Dec 26.
Overdose of Cu is associated with multiple diseases, such as Wilson disease, Parkinson disease, and Alzheimer disease. Therefore, detections of Cu in vivo and in vitro are meaningful. Near-infrared fluorescent probes are commonly applied for the detection of Cu in real time. With mono detection signal provided, these probes are not persuasive when applied to complicated biological environments, such as cells and animals. In this report, we conjugated the organic fluorescent probe CYDAC with the UCNPs (lanthanide-doped upconversion nanoparticles) to develop a ratiometric luminescent probe for Cu. The composite material UCNPs-CYDAC provides a ratiometric signal based on an upconversion luminescence 660 and 800 nm. With good sensitivity and selectivity for Cu, it works well in vivo and in vitro for the detection of Cu. We believe it is promising in further biological applications.
铜过量与多种疾病有关，如威尔逊病、帕金森病和阿尔茨海默病。因此，体内和体外的铜检测具有重要意义。近红外荧光探针常用于实时检测铜。这些探针提供单一的检测信号，在应用于复杂的生物环境，如细胞和动物时，说服力不够。在本报告中，我们将有机荧光探针 CYDAC 与上转换纳米粒子（镧系掺杂上转换纳米粒子）偶联，开发了一种用于铜的比率发光探针。复合材料 UCNPs-CYDAC 提供了基于上转换发光 660nm 和 800nm 的比率信号。该探针对铜具有良好的灵敏度和选择性，可在体内和体外很好地检测铜。我们相信它在进一步的生物应用中很有前景。
