Van Handel E
Institute of Food and Agricultural Sciences, University of Florida, Vero Beach 32962.
J Am Mosq Control Assoc. 1985 Sep;1(3):302-4.
Total lipids in individual mosquitoes can be determined by extraction with chloroform-methanol followed by reaction with sulfuric acid and a vanillin-phosphoric acid reagent. By subtracting the lipids determined in a starved population, the method is suitable to establish variations of lipid reserves in field populations.
单个蚊子体内的总脂质可以通过用氯仿 - 甲醇萃取，然后与硫酸和香草醛 - 磷酸试剂反应来测定。通过减去饥饿群体中测定的脂质，该方法适用于确定野外群体中脂质储备的变化。
