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蚊子中总脂质的快速测定

Rapid determination of total lipids in mosquitoes.

作者信息

Van Handel E

机构信息

Institute of Food and Agricultural Sciences, University of Florida, Vero Beach 32962.

出版信息

J Am Mosq Control Assoc. 1985 Sep;1(3):302-4.

PMID:2906672
Abstract

Total lipids in individual mosquitoes can be determined by extraction with chloroform-methanol followed by reaction with sulfuric acid and a vanillin-phosphoric acid reagent. By subtracting the lipids determined in a starved population, the method is suitable to establish variations of lipid reserves in field populations.

摘要

单个蚊子体内的总脂质可以通过用氯仿 - 甲醇萃取,然后与硫酸和香草醛 - 磷酸试剂反应来测定。通过减去饥饿群体中测定的脂质,该方法适用于确定野外群体中脂质储备的变化。

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