Laboratorio de Variación Biológica y Evolución, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Prolongación de Carpio y Plan de Ayala s/n. Delegación Miguel Hidalgo, CP11340, México, DF, Mexico.
Laboratorio de Ecología Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n. Delegación Miguel Hidalgo, CP11340, Mexico, DF, Mexico.
Sci Rep. 2017 Oct 24;7(1):13864. doi: 10.1038/s41598-017-14031-6.
Dendroctonus bark beetles comprise 20 taxonomically recognized species, which are one of the most destructive pine forest pests in North and Central America, and Eurasia. The aims of this study were to characterize the gut bacterial diversity, to determine the core bacteriome and to explore the ecological association between these bacteria and bark beetles. A total of five bacterial phyla were identified in the gut of 13 Dendroctonus species; Proteobacteria was the most abundant, followed by Firmicutes, Fusobacteria, Actinobacteria and Deinococcus-Thermus. The α-diversity was low as demonstrated in previous studies and significant differences in β-diversity were observed. The core bacteriome was composed of Enterobacter, Pantoea, Pseudomonas, Rahnella, Raoultella, and Serratia. The tanglegram between bacteria and bark beetles suggests that members of bacterial community are acquired from the environment, possibly from the host tree. These findings improve the knowledge about the bacterial community composition, and provide the bases to study the metabolic functions of these bacteria, as well as their interaction with these bark beetles.
枝梢小蠹包含 20 个已被分类确认的物种，是北美洲和中美洲及欧亚大陆地区松林最具破坏力的害虫之一。本研究旨在描述肠道细菌多样性，确定核心细菌组，并探索这些细菌与小蠹虫之间的生态联系。在 13 种枝梢小蠹的肠道中共鉴定出 5 个细菌门；变形菌门是最丰富的，其次是厚壁菌门、梭杆菌门、放线菌门和异常球菌-栖热菌门。α-多样性与之前的研究结果一样低，β-多样性存在显著差异。核心细菌组由肠杆菌属、泛菌属、假单胞菌属、拉恩菌属、罗尔氏菌属和沙雷氏菌属组成。细菌与小蠹虫之间的缠结图表明，细菌群落成员是从环境中获得的，可能来自于宿主树。这些发现提高了对细菌群落组成的认识，并为研究这些细菌的代谢功能以及它们与这些小蠹虫的相互作用提供了基础。
