Prell G D, Khandelwal J K, Burns R S, LeWitt P A, Green J P
Department of Pharmacology, Mount Sinai School of Medicine, City University of New York.
Compr Gerontol A. 1988 Oct;2(3):114-9.
The metabolites of histamine, tele-methylhistamine (t-MH) and tele-methylimidazoleacetic acid (t-MIAA), were measured in lumbar cerebrospinal fluid of healthy, normal volunteers aged 20-31 (n = 4) and 60-72 (n = 8) by gas chromatography-mass spectrometry. Mean levels (pmol/ml) of t-MH, t-MIAA and the sum of t-MH and t-MIAA (2.9, 6.4 and 9.4, respectively) were significantly higher in CSF from older subjects than from younger subjects (1.1, 4.5 and 5.5, respectively). Another older subject had yet higher levels of metabolites (6.7, 15.1 and 21.8, respectively). The sum of the levels of the known metabolites of histamine in brain, i.e. t-MH and t-MIAA, did not overlap between the younger and older subjects. The levels of pros-methylimidazoleacetic acid, an endogenous isomer of t-MIAA that is not derived from metabolism of histamine, did not differ significantly between the two groups. These findings contrast with results of similar studies of metabolites of other aminergic transmitters in showing elevated levels of metabolites of histamine in cerebrospinal fluid with increasing age.
通过气相色谱-质谱联用技术，对年龄在20 - 31岁（n = 4）和60 - 72岁（n = 8）的健康正常志愿者的腰椎脑脊液中的组胺代谢产物——N-甲基组胺（t-MH）和N-甲基咪唑乙酸（t-MIAA）进行了测定。老年受试者脑脊液中t-MH、t-MIAA以及t-MH与t-MIAA之和的平均水平（pmol/ml）（分别为2.9、6.4和9.4）显著高于年轻受试者（分别为1.1、4.5和5.5）。另一位老年受试者的代谢产物水平更高（分别为6.7、15.1和21.8）。年轻和老年受试者之间，大脑中已知的组胺代谢产物（即t-MH和t-MIAA）水平之和没有重叠。t-MIAA的内源性异构体——前甲基咪唑乙酸的水平，该异构体并非来自组胺代谢，两组之间没有显著差异。这些发现与其他胺能递质代谢产物的类似研究结果形成对比，表明随着年龄增长，脑脊液中组胺代谢产物水平升高。
