Tian Rui, Sun Jiaheng, Qi Yanfei, Zhang Boyu, Guo Shuanli, Zhao Mingming
School of Public Health, Jilin University, Changchun 130021, Jilin, China.
Nanomaterials (Basel). 2017 Oct 25;7(11):347. doi: 10.3390/nano7110347.
Nanozyme-based colorimetric sensors have received considerable attention due to their unique properties. The size, shape, and surface chemistry of these nanozymes could dramatically influence their sensing behaviors. Herein, a comparative study of VO₂ nanoparticles with different morphologies (nanofibers, nanosheets, and nanorods) was conducted and applied to the sensitive colorimetric detection of H₂O₂ and glucose. The peroxidase-like activities and mechanisms of VO₂ nanoparticles were analyzed. Among the VO₂ nanoparticles, VO₂ nanofibers exhibited the best peroxidase-like activity. Finally, a comparative quantitative detections of H₂O₂ and glucose were done on fiber, sheet, and rod nanoparticles. Under the optimal reaction conditions, the lower limit of detection (LOD) of the VO₂ nanofibers, nanosheets, and nanorods for H₂O₂ are found to be 0.018, 0.266, and 0.41 mM, respectively. The VO₂ nanofibers, nanosheets, and nanorods show the linear response for H₂O₂ from 0.025-10, 0.488-62.5, and 0.488-15.625 mM, respectively. The lower limit of detection (LOD) of the VO₂ nanofibers, nanosheets, and nanorods for glucose are found to be 0.009, 0.348, and 0.437 mM, respectively. The VO₂ nanofibers, nanosheets, and nanorods show the linear response for glucose from 0.01-10, 0.625-15, and 0.625-10 mM, respectively. The proposed work will contribute to the nanozyme-based colorimetric assay.
基于纳米酶的比色传感器因其独特性能而备受关注。这些纳米酶的尺寸、形状和表面化学性质会显著影响其传感行为。在此，对不同形貌（纳米纤维、纳米片和纳米棒）的VO₂纳米颗粒进行了对比研究，并将其应用于H₂O₂和葡萄糖的灵敏比色检测。分析了VO₂纳米颗粒的类过氧化物酶活性及机制。在VO₂纳米颗粒中，VO₂纳米纤维表现出最佳的类过氧化物酶活性。最后，对纤维状、片状和棒状纳米颗粒进行了H₂O₂和葡萄糖的对比定量检测。在最佳反应条件下，发现VO₂纳米纤维、纳米片和纳米棒对H₂O₂的检测下限（LOD）分别为0.018、0.266和0.41 mM。VO₂纳米纤维、纳米片和纳米棒对H₂O₂的线性响应范围分别为0.025 - 10、0.488 - 62.5和0.488 - 15.625 mM。VO₂纳米纤维、纳米片和纳米棒对葡萄糖的检测下限（LOD）分别为0.009、0.348和0.437 mM。VO₂纳米纤维、纳米片和纳米棒对葡萄糖的线性响应范围分别为0.01 - 10、0.625 - 15和0.625 - 10 mM。所提出的工作将有助于基于纳米酶的比色测定。
