Sun Ruimeng, Lv Ruijuan, Zhang Yang, Du Ting, Li Yuhan, Chen Lixia, Qi Yanfei
School of Public Health, Jilin University Changchun 130021 Jilin P. R. China
RSC Adv. 2022 Aug 9;12(34):21875-21884. doi: 10.1039/d2ra02375j. eCollection 2022 Aug 4.
The catalytic properties of bimetallic nanoparticles have been widely studied by researchers in many fields. In this paper, core-shell Cu/Au nanoparticles (Cu/Au NPs) were synthesized by a simple and mild one-pot method, and their peroxidase activity was proved by catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) with color change to blue. The change of solution color and absorbance strongly depends on the concentration of HO, so it can be used for direct detection of HO and indirect detection of glucose. What's more, GSH can efficiently react with the hydroxyl radicals from HO catalyzed by core-shell Cu/Au NPs to inhibit the production of ox-TMB. Thus, the concentration of GSH can be determined by the decrease in the absorbance of the solution at 652 nm. The results showed that our proposed strategy had good detection range and detection limit for the detection of glucose and GSH. This method has been used in the detection of practical samples and has great application potential in environmental monitoring and clinical diagnosis.
双金属纳米颗粒的催化性能已被许多领域的研究人员广泛研究。在本文中，通过一种简单温和的一锅法合成了核壳结构的铜/金纳米颗粒（Cu/Au NPs），并通过催化3,3',5,5'-四甲基联苯胺（TMB）氧化使其颜色变为蓝色，证明了其过氧化物酶活性。溶液颜色和吸光度的变化强烈依赖于过氧化氢（HO）的浓度，因此它可用于直接检测HO和间接检测葡萄糖。此外，谷胱甘肽（GSH）可以与核壳Cu/Au NPs催化HO产生的羟基自由基有效反应，抑制氧化型TMB的产生。因此，可以通过溶液在652nm处吸光度的降低来测定GSH的浓度。结果表明，我们提出的策略在检测葡萄糖和GSH方面具有良好的检测范围和检测限。该方法已用于实际样品的检测，在环境监测和临床诊断中具有巨大的应用潜力。
