Wang Ting, Zhang Zhiwei, Xing Huaixin, Wang Li, Zhang Guoxiang, Yu Na, Wang Junzhi, Guo Wei, Jiang Jie
Department of Obstetrics and Gynecology, Shandong Provincial Qianfoshan Hospital Department of Anesthesiology, Shandong Cancer Hospital Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, P.R. China.
Medicine (Baltimore). 2017 Oct;96(43):e8359. doi: 10.1097/MD.0000000000008359.
This study is conducted to investigate the involvement of T-helper (Th) cells and regulatory T cells in epithelial ovarian cancer (EOC).The percentages of Th22, Th17, Th1, and regulatory T cells in the peripheral blood of EOC patients, benign ovarian epithelial neoplasm (BOEN) patients, and healthy control (HC) were examined by flow cytometry. Enzyme-linked immunosorbent assay was used to determine serum levels of interleukin (IL)-22, IL-17, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α).Th22 and Th17 were significantly increased in EOC patients. The plasma concentrations of IL-22 and TNF-α were significantly elevated in EOC patients compared with BOEN patients and HC. In EOC patients, there was an increased trend of Th22, IL-22, and TNF-α in stage III-IV patients compared with stage I-II patients. A positive correlation was seen among Th22, Th17, and Th1 cells in EOC patients. Similarly, positive correlations were detected between Th22 cells and IL-22 or TNF-α and between Th1 cells and interferon-γ (IFN-γ) in EOC patients. Besides, no significant difference was found in Th1 cells and regulatory T cells among EOC and BOEN patients and HC.There is a higher circulating frequency of Th22, Th17 cells, IL-22, and TNF-α concentration in EOC patients, which may conjointly participate in the pathogenesis and growth of EOC.
本研究旨在探讨辅助性T（Th）细胞和调节性T细胞在上皮性卵巢癌（EOC）中的作用。采用流式细胞术检测EOC患者、良性卵巢上皮性肿瘤（BOEN）患者及健康对照（HC）外周血中Th22、Th17、Th1和调节性T细胞的百分比。采用酶联免疫吸附测定法测定血清白细胞介素（IL）-22、IL-17、干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）水平。EOC患者中Th22和Th17显著增加。与BOEN患者和HC相比，EOC患者血浆中IL-22和TNF-α浓度显著升高。在EOC患者中，Ⅲ-Ⅳ期患者的Th22、IL-22和TNF-α较Ⅰ-Ⅱ期患者有升高趋势。EOC患者中Th22、Th17和Th1细胞之间呈正相关。同样，EOC患者中Th22细胞与IL-22或TNF-α之间以及Th1细胞与干扰素-γ（IFN-γ）之间呈正相关。此外，EOC患者、BOEN患者和HC之间的Th1细胞和调节性T细胞无显著差异。EOC患者中Th22、Th17细胞的循环频率较高，IL-22和TNF-α浓度较高，它们可能共同参与EOC的发病机制和生长过程。
