Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
Mol Cancer. 2017 Oct 25;16(1):166. doi: 10.1186/s12943-017-0735-3.
Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play a crucial role in tumorigenesis. Here, we report a novel lncRNA, RP11-436H11.5, that regulates renal cell carcinoma (RCC) cell proliferation and invasion by sponging miR-335-5p.
Expression of lncRNA RP11-436H11.5 was determined by a qRT-PCR assay in RCC tissues. RCC cell proliferation and invasion were measured by a cell proliferation assay and a transwell invasion assay. Expression of BCL-W was detected by a western blot assay. Interactions between lncRNA RP11-436H11.5 and miR-335-5p were measured by a luciferase reporter assay and a RNA-pull down assay. In vivo experiments were used to detect tumor formation.
In this study, the qRT-PCR results illustrated that lncRNA RP11-436H11.5 was more highly expressed in RCC tissues than in adjacent normal renal tissues. The results of survival analysis indicated that patients in the high lncRNA RP11-436H11.5 group presented significantly worse outcomes compared with those in the low lncRNA RP11-436H11.5 group. Downregulation of lncRNA RP11-436H11.5 suppressed RCC cell proliferation and invasion in vitro and in vivo. Luciferase reporter assay results demonstrated that lncRNA RP11-436H11.5 enhanced BCL-W expression by regulating miR-335-5p expression. LncRNA RP11-436H11.5 could function as a miR-335-5p decoy to derepress expression of BCL-W.
LncRNA RP11-436H11.5 could function as a competing endogenous RNA to promote RCC cell proliferation and invasion, which might serve as a therapeutic application to suppress RCC progression.
越来越多的证据表明，长非编码 RNA（lncRNA）在肿瘤发生中起着至关重要的作用。在这里，我们报告了一种新的 lncRNA，RP11-436H11.5，它通过海绵 miR-335-5p 来调节肾细胞癌（RCC）细胞的增殖和侵袭。
通过 qRT-PCR 检测 RP11-436H11.5 在 RCC 组织中的表达。通过细胞增殖测定和 Transwell 侵袭测定来测量 RCC 细胞的增殖和侵袭。通过 Western blot 检测 BCL-W 的表达。通过荧光素酶报告测定和 RNA 下拉测定来测量 lncRNA RP11-436H11.5 与 miR-335-5p 之间的相互作用。体内实验用于检测肿瘤形成。
本研究的 qRT-PCR 结果表明，lncRNA RP11-436H11.5 在 RCC 组织中的表达高于相邻正常肾组织。生存分析结果表明，lncRNA RP11-436H11.5 高表达组患者的预后明显差于 lncRNA RP11-436H11.5 低表达组患者。体外和体内下调 lncRNA RP11-436H11.5 抑制 RCC 细胞的增殖和侵袭。荧光素酶报告测定结果表明，lncRNA RP11-436H11.5 通过调节 miR-335-5p 的表达增强 BCL-W 的表达。lncRNA RP11-436H11.5 可以作为 miR-335-5p 的诱饵来解除 BCL-W 的表达抑制。
lncRNA RP11-436H11.5 可以作为竞争性内源性 RNA 促进 RCC 细胞的增殖和侵袭，这可能成为抑制 RCC 进展的治疗应用。
