Lü M-H, Tang B, Zeng S, Hu C-J, Xie R, Wu Y-Y, Wang S-M, He F-T, Yang S-M
Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
Department of Gastroenterology, The Affiliated Hospital of Luzhou Medical College, Luzhou, China.
Oncogene. 2016 Jul 7;35(27):3524-34. doi: 10.1038/onc.2015.413. Epub 2015 Nov 9.
Long noncoding RNAs (lncRNAs) are emerging as new players in gene regulation and are associated with the development of cancers. To investigate the important role and mechanism of lncRNAs in the progression of gastric cancer, we screened lncRNAs in gastric cancer tissues and corresponding adjacent tissues, and assessed the effects on gastric cancer. Here, we report that BC032469, a novel lncRNA, expressed highly in gastric cancer tissues, and the upregulation was clinically associated with larger tumor size, poor differentiation and shorter survival of gastric cancer patients. Downregulation of BC032469 resulted in a significant inhibition of proliferation in vitro and in vivo. Mechanistically, BC032469 could directly bind to miR-1207-5p and effectively functioned as a sponge for miR-1207-5p to modulate the derepression of hTERT. Thus, BC032469 may function as a ceRNA to impair miR-1207-5p-dependent hTERT downregulation, suggesting that it may be clinically valuable as a poor prognostic biomarker of gastric cancer.
长链非编码RNA（lncRNAs）正成为基因调控中的新角色，并与癌症的发展相关。为了研究lncRNAs在胃癌进展中的重要作用和机制，我们在胃癌组织及相应的癌旁组织中筛选lncRNAs，并评估其对胃癌的影响。在此，我们报道了一种新型lncRNA BC032469，其在胃癌组织中高表达，且这种上调在临床上与胃癌患者更大的肿瘤大小、低分化及较短生存期相关。BC032469的下调导致体内外增殖的显著抑制。机制上，BC032469可直接与miR-1207-5p结合，并有效地作为miR-1207-5p的海绵发挥作用，以调节hTERT的去抑制。因此，BC032469可能作为一种竞争性内源性RNA（ceRNA）来削弱miR-1207-5p依赖的hTERT下调，这表明它作为胃癌预后不良的生物标志物可能具有临床价值。
