Intharanut Kamphon, Bejrachandra Sasitorn, Nathalang Siriporn, Leetrakool Nipapan, Nathalang Oytip
Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand.
National Blood Centre, Thai Red Cross Society, Bangkok, Thailand.
Transfus Med Hemother. 2017 Sep;44(5):358-364. doi: 10.1159/000471886. Epub 2017 May 12.
Antigen-negative red cell transfusion is required for transfusion-dependent patients. We developed multiplex PCR for red cell genotyping and calculated the possibility of finding compatible predicted phenotypes in Thai blood donor populations according to red cell alloantibodies found among Thai patients.
600 DNA samples obtained from unrelated healthy central and northern Thai blood donors were tested with the newly developed multiplex PCR for , , , , , , and , and allele detections. Additionally, the possibility of finding compatible predicted phenotypes in two Thai blood donor populations was calculated to estimate the minimal number of tests needed to provide compatible blood.
The validity of multiplex PCR using known DNA controls and the phenotyping and genotyping results obtained by serological and PCR-SSP techniques were in agreement. The possibility of finding at least one compatible blood unit for patients with multiple antibodies was comparable in Thai populations.
The multiplex PCR for red cell genotyping simultaneously interprets 7 alleles and 1 hybrid GP group. Similar strategies can be applied in other populations depending on alloantibody frequencies in transfusion-dependent patients, especially in a country with limited resources.
依赖输血的患者需要输注抗原阴性的红细胞。我们开发了用于红细胞基因分型的多重聚合酶链反应（PCR），并根据泰国患者中发现的红细胞同种抗体，计算了在泰国献血人群中找到相容预测表型的可能性。
从泰国中部和北部无亲缘关系的健康献血者中获取600份DNA样本，使用新开发的多重PCR对 、 、 、 、 、 以及 和 等位基因进行检测。此外，计算了在两个泰国献血人群中找到相容预测表型的可能性，以估计提供相容血液所需的最少检测次数。
使用已知DNA对照的多重PCR的有效性与通过血清学和PCR-序列特异性引物（PCR-SSP）技术获得的表型和基因分型结果一致。在泰国人群中，为具有多种抗体的患者找到至少一个相容血液单位的可能性相当。
用于红细胞基因分型的多重PCR可同时解读7个等位基因和1个杂合糖蛋白（GP）组。根据依赖输血患者的同种抗体频率，类似策略可应用于其他人群，尤其是在资源有限的国家。
