Eroshenko Galina A, Nosov Nikita Yu, Krasnov Yaroslav M, Oglodin Yevgeny G, Kukleva Lyubov M, Guseva Natalia P, Kuznetsov Alexander A, Abdikarimov Sabyrzhan T, Dzhaparova Aigul K, Kutyrev Vladimir V
Russian Research Anti-Plague Institute "Microbe", Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare, Saratov, Russian Federation.
Center for Quarantine and Particularly Dangerous Infections, Department of Disease Prevention of the Ministry of Health of the Kyrgyz Republic, Bishkek, the Kyrgyz Republic.
PLoS One. 2017 Oct 26;12(10):e0187230. doi: 10.1371/journal.pone.0187230. eCollection 2017.
Fifty six Yersinia pestis strains, isolated over the period of more than 50 years in three high-mountain foci of Kyrgyzstan (Tien Shan, Alai, and Talas), have been characterized by means of PCR and single nucleotide polymorphism (SNP) typing methods. Seven of these strains were also characterized by means of whole genome sequencing and genome-wide SNP phylogenetic analysis. It was found that forty two strains belong to 0.ANT2, 0.ANT3 and 0.ANT5 phylogenetic branches. From these, strains of 0.ANT2 and 0.ANT3 branches were earlier detected in China only, whereas 0.ANT5 phylogenetic branch was identified for Y. pestis phylogeny for the first time. According to the results of genome-wide SNP analysis, 0.ANT5 strains are ones of the most closely related to Y. pestis strain responsible for the Justinianic Plague. We have also found out that four of the studied strains belong to the phylogenetic branch 2.MED1, and ten strains from Talas high-mountain focus belong to the phylogenetic branch 0.PE4 (sub-branch 0.PE4t). Established diversity of Y. pestis strains and extensive dissemination of the strains pertaining to the 0.ANT branch confirm the antiquity of the mentioned above plague foci and suggest that strains of the 0.ANT branch, which serve as precursors for all highly virulent Y. pestis strains, had their origin in the Tien Shan mountains.
在吉尔吉斯斯坦的三个高山疫源地（天山、阿赖和塔拉斯），历经50多年分离出56株鼠疫耶尔森菌菌株，已通过聚合酶链反应（PCR）和单核苷酸多态性（SNP）分型方法进行了特征鉴定。其中7株还通过全基因组测序和全基因组SNP系统发育分析进行了特征鉴定。结果发现，42株属于0.ANT2、0.ANT3和0.ANT5系统发育分支。其中，0.ANT2和0.ANT3分支的菌株此前仅在中国被检测到，而0.ANT5系统发育分支是首次在鼠疫耶尔森菌系统发育中被鉴定出来。根据全基因组SNP分析结果，0.ANT5菌株是与导致查士丁尼鼠疫的鼠疫耶尔森菌菌株关系最密切的菌株之一。我们还发现，所研究的菌株中有4株属于系统发育分支2.MED1，来自塔拉斯高山疫源地的10株菌株属于系统发育分支0.PE4（0.PE4t亚分支）。鼠疫耶尔森菌菌株的多样性以及0.ANT分支菌株的广泛传播证实了上述鼠疫疫源地的古老性，并表明作为所有高毒力鼠疫耶尔森菌菌株前身的0.ANT分支菌株起源于天山山脉。
