School of Life Science, Liaoning University, Shenyang, Chongshan Middle Road 66, Huanggu District, Shenyang, Liaoning, 110036, China.
Liaoning Forestry Academy, Shenyang, 110032, China.
Plant Foods Hum Nutr. 2017 Dec;72(4):425-431. doi: 10.1007/s11130-017-0644-z.
Liver ageing is a significant risk factor for chronic liver diseases. Anthocyanin is a food additive that has previously shown efficacy in increasing longevity. Here, we tested whether anthocyanins could protect young mice from accelerated ageing of the liver. Kunming mice were injected with D-galactose to accelerate ageing and were given 20 or 40 mg/kg anthocyanins as an intervention. After eight weeks, whole liver function and structure were evaluated, and the expression levels of genes involved in the DNA damage signalling pathway were assessed by Western blot analysis. Anthocyanins delayed the reduction of the liver index (p < 0.05), hepatic tissue injury and fibrosis. Anthocyanins also maintained the stability of the redox system (GSH-PX, T-SOD and MDA) in plasma and liver structures (p < 0.001) and reduced the levels of inflammatory factors (IL-1, IL-6 and TNF-α) in the liver (p < 0.05). Moreover, the expression levels of sensors (ATM and ATR), mediators (H2AX and γ-H2AX) and effectors (Chk1, Chk2, p53 and p-p53) in the DNA damage signalling pathway were all reduced. Anthocyanins could be widely used in the field of health products to slow ageing-related deterioration of liver function and structure by inhibiting DNA damage.
肝脏衰老（ageing）是慢性肝病的重要危险因素。花色苷（Anthocyanin）是一种食品添加剂，先前已显示出能延长寿命的功效。在这里，我们测试了花色苷是否可以保护年轻小鼠免受肝脏加速衰老的影响。昆明小鼠被注射半乳糖以加速衰老，并给予 20 或 40mg/kg 的花色苷作为干预。八周后，评估了整个肝脏的功能和结构，并通过 Western blot 分析评估了参与 DNA 损伤信号通路的基因的表达水平。花色苷延缓了肝指数的降低（p<0.05）、肝组织损伤和纤维化。花色苷还维持了氧化还原系统（GSH-PX、T-SOD 和 MDA）在血浆和肝结构中的稳定性（p<0.001），并降低了肝脏中炎症因子（IL-1、IL-6 和 TNF-α）的水平（p<0.05）。此外，DNA 损伤信号通路中的传感器（ATM 和 ATR）、介质（H2AX 和 γ-H2AX）和效应器（Chk1、Chk2、p53 和 p-p53）的表达水平均降低。花色苷可以广泛应用于保健品领域，通过抑制 DNA 损伤来延缓与衰老相关的肝功能和结构恶化。
