Sallem Fadoua, Boudon Julien, Heintz Olivier, Séverin Isabelle, Megriche Adel, Millot Nadine
Université de Tunis El Manar, Faculté des Sciences de Tunis, UR11ES18 Chimie Minérale Appliquée, Campus Universitaire Farhat Hached, 2092, Tunis, Tunisia.
Dalton Trans. 2017 Nov 14;46(44):15386-15398. doi: 10.1039/c7dt03029k.
In this work, we discuss for the first time the elaboration of nanohybrid materials, intended for drug delivery systems, based on titanate nanotubes (TiONts) coated with chitosan polymer (CT). Chitosan has been used to enhance the biocompatibility of hydrothermally synthesized nanotubes in biological medium as a substitute for the polyethylene glycol (PEG) that is generally used for biocompatibility. CT grafting was carried out using two different approaches; the first was made by a covalent bond using two intermediate molecules, and the second is based on electrostatic interactions between CT and TiONts. The type of elaborated bond on the surface of TiONts was proven to influence the colloidal stability of the elaborated nanohybrids, which were studied in different media. A detailed comparison between these two approaches was carried by XPS and TGA-SM techniques. Finally, an original and sensitive cytotoxicity assay consisting of the measurement of the cells' total RNA synthesis was used to prove the non-toxicity of both obtained nanohybrids.
在这项工作中，我们首次讨论了基于包覆壳聚糖聚合物（CT）的钛酸盐纳米管（TiONts）制备用于药物递送系统的纳米杂化材料。壳聚糖已被用于提高水热合成纳米管在生物介质中的生物相容性，以替代通常用于生物相容性的聚乙二醇（PEG）。CT接枝采用两种不同的方法进行；第一种是通过共价键使用两个中间分子，第二种基于CT与TiONts之间的静电相互作用。事实证明，TiONts表面形成的键的类型会影响所制备纳米杂化物在不同介质中的胶体稳定性。通过XPS和TGA-SM技术对这两种方法进行了详细比较。最后，使用一种原始且灵敏的细胞毒性测定法，即测量细胞的总RNA合成，来证明所获得的两种纳米杂化物均无毒。
