高通量方法在细胞行为筛选和分析中的应用。
High-throughput approaches for screening and analysis of cell behaviors.
机构信息
Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA; Center for Biomaterials, Korea Institute of Science and Technology, 14 Hwarang-ro, Seongbuk-gu, Seoul, 02792, South Korea.
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA.
出版信息
Biomaterials. 2018 Jan;153:85-101. doi: 10.1016/j.biomaterials.2017.06.022. Epub 2017 Jun 21.
The rapid development of new biomaterials and techniques to modify them challenge our capability to characterize them using conventional methods. In response, numerous high-throughput (HT) strategies are being developed to analyze biomaterials and their interactions with cells using combinatorial approaches. Moreover, these systematic analyses have the power to uncover effects of delivered soluble bioactive molecules on cell responses. In this review, we describe the recent developments in HT approaches that help identify cellular microenvironments affecting cell behaviors and highlight HT screening of biochemical libraries for gene delivery, drug discovery, and toxicological studies. We also discuss HT techniques for the analyses of cell secreted biomolecules and provide perspectives on the future utility of HT approaches in biomedical engineering.
新的生物材料和修饰它们的技术的快速发展挑战了我们使用传统方法对其进行表征的能力。为此，正在开发许多高通量 (HT) 策略，以使用组合方法分析生物材料及其与细胞的相互作用。此外，这些系统分析具有揭示递送到细胞中的可溶性生物活性分子对细胞反应的影响的能力。在这篇综述中，我们描述了有助于识别影响细胞行为的细胞微环境的 HT 方法的最新进展，并强调了用于基因传递、药物发现和毒理学研究的生物化学文库的 HT 筛选。我们还讨论了用于分析细胞分泌的生物分子的 HT 技术，并对 HT 方法在生物医学工程中的未来应用提供了一些看法。
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