School of Electrical and Electronic Engineering, Yonsei University, Seoul, 03722, Korea.
Curr Pharm Des. 2018;24(45):5458-5470. doi: 10.2174/1381612825666190207093438.
In the last decades, bioengineers have developed myriad biomaterials for regenerative medicine. Development of screening techniques is essential for understanding complex behavior of cells in the biological microenvironments. Conventional approaches to the screening of cellular behavior in vitro have limitations in terms of accuracy, reusability, labor-intensive screening, and versatility. Thus, drug screening and toxicology test through in vitro screening platforms have been underwhelming. Recent advances in the high-throughput screening platforms somewhat overcome the limitations of in vitro screening platforms via repopulating human tissues' biophysical and biomchemical microenvironments with the ability to continuous monitoring of miniaturized human tissue behavior. Herein, we review current trends in the screening platform in which a high-throughput system composed of engineered microarray devices is developed to investigate cell-biomaterial interaction. Furthermore, diverse methods to achieve continuous monitoring of cell behavior via developments of biosensor integrated high-throughput platforms, and future perspectives on high-throughput screening will be provided.
在过去的几十年中，生物工程师已经开发出了无数种用于再生医学的生物材料。开发筛选技术对于理解细胞在生物微环境中的复杂行为至关重要。传统的体外细胞行为筛选方法在准确性、可重复性、劳动密集型筛选和多功能性方面存在局限性。因此，通过体外筛选平台进行药物筛选和毒理学测试的效果并不理想。高通量筛选平台的最新进展在一定程度上通过用能够连续监测微型人体组织行为的能力来重新填充人体组织的生物物理和生物化学微环境，克服了体外筛选平台的局限性。本文综述了目前的筛选平台趋势，其中开发了由工程微阵列设备组成的高通量系统来研究细胞-生物材料相互作用。此外，还提供了通过开发集成生物传感器的高通量平台实现对细胞行为的连续监测的各种方法，以及高通量筛选的未来展望。
