Rusanov A L, Romashin D D, Zgoda V G, Butkova T V, Luzgina N G
V. N. Orekhovich Institute of Biomedical Chemistry, Russian Federation.
Data Brief. 2021 Feb 13;35:106871. doi: 10.1016/j.dib.2021.106871. eCollection 2021 Apr.
Learning of the molecular mechanisms of the pathological processes development in the normal human keratinocytes (NHK) are difficult. Immortalized keratinocytes HaCaT are often used as an analogue of NHK since they have a number of advantages over the latter - they do not require the presence of growth and differentiation factors in the medium, have unlimited potential for proliferation, demonstrate stable phenotype regardless of the number of passages [1]. Taking into account the properties and characteristics of the HaCaT line, these cells can be considered as a promising experimental model for research of various physiological processes occurring in human keratinocytes. However, to understand the limitations of such an experimental model, a detailed comparative characterization of HaCaT and NHK is required, which can be obtained by carrying out its proteomic analysis. In this article we present datasets obtained through the high-throughput shotgun proteomics analysis of normal human keratinocytes and immortalized HaCaT keratinocytes. As a protocol for proteomic profiling of cells, we used the approach of obtaining LC-MS / MS measurements followed by their processing with Progenesis LC-MS software (Nonlinear Dynamics Ltd.). The mzML files were deposited to the Mendeley Data.
了解正常人角质形成细胞（NHK）病理过程发展的分子机制颇具难度。永生化角质形成细胞HaCaT常被用作NHK的类似物，因为它们相较于NHK具有诸多优势——它们在培养基中无需生长和分化因子的存在，具有无限增殖潜力，无论传代次数多少都表现出稳定的表型[1]。考虑到HaCaT细胞系的特性和特征，这些细胞可被视为研究人类角质形成细胞中各种生理过程的有前景的实验模型。然而，为了理解这种实验模型的局限性，需要对HaCaT和NHK进行详细的比较表征，这可以通过对其进行蛋白质组学分析来获得。在本文中，我们展示了通过对正常人角质形成细胞和永生化HaCaT角质形成细胞进行高通量鸟枪法蛋白质组学分析获得的数据集。作为细胞蛋白质组学分析的方案，我们采用了先获得LC-MS / MS测量值，然后用Progenesis LC-MS软件（非线性动力学有限公司）对其进行处理的方法。mzML文件已存入Mendeley数据。
