Bauman Cathy A, Jones-Bitton Andria, Ahlstrom Christina, Mutharia Lucy, De Buck Jeroen, Jansen Jocelyn, Kelton David, Menzies Paula
Department of Population Medicine, University of Guelph, Ontario N1G 2W1 (Bauman, Jones-Bitton, Kelton, Menzies); Department of Production Animal Health, University of Calgary, Alberta T2N 1N4 (Ahlstrom, De Buck); Department of Molecular and Cellular Biology, University of Guelph, Ontario N1G 2W1 (Mutharia); Veterinary Science Unit, Animal Health and Welfare Branch, Ontario Ministry of Agriculture, Food and Rural Affairs (Jansen).
Can J Vet Res. 2017 Oct;81(4):304-307.
The main objective of this study was to identify the circulating strains of subspecies (Map) in fecal isolates obtained from dairy goat (N = 29 farms) and dairy sheep (N = 21 farms) populations in Ontario, Canada. Further subtyping was performed to determine if there was adequate diversity between strains that could be used to establish Map transmission patterns. Type C was the dominant strain of Map isolates (95.2%) identified in dairy goats ( = 21). Sub-typing of the Type C strains, based on variable number tandem repeats (VNTR) and mycobacterial interspersed repetitive units, identified 3 VNTR types: INMV 1 ( = 10), INMV 2 ( = 10), and a type not previously identified ( = 1). Only 2 sheep isolates could be identified; both were Type S, sub-type III. Current typing methods demonstrate little Map diversity in the dairy goat population and are therefore of limited use to investigate infection patterns.
本研究的主要目的是在加拿大安大略省的奶山羊（N = 29个养殖场）和奶绵羊（N = 21个养殖场）群体的粪便分离物中鉴定副结核分枝杆菌亚种（Map）的流行菌株。进行了进一步的亚型分析，以确定菌株之间是否存在足够的多样性，可用于建立Map的传播模式。C型是在奶山羊（n = 21）中鉴定出的Map分离株的主要菌株（95.2%）。基于可变数目串联重复序列（VNTR）和分枝杆菌散布重复单元对C型菌株进行亚型分析，鉴定出3种VNTR类型：INMV 1（n = 10）、INMV 2（n = 10）和一种先前未鉴定的类型（n = 1）。仅鉴定出两株绵羊分离株；均为S型，III亚型。目前的分型方法表明奶山羊群体中Map的多样性很小，因此在调查感染模式方面用途有限。
