Kook Yun-Min, Jeong Yoon, Lee Kangwon, Koh Won-Gun
Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea.
Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.
J Tissue Eng. 2017 Aug 18;8:2041731417724640. doi: 10.1177/2041731417724640. eCollection 2017 Jan-Dec.
The extracellular matrix of most natural tissues comprises various types of cells, including fibroblasts, stem cells, and endothelial cells, which communicate with each other directly or indirectly to regulate matrix production and cell functionality. To engineer multicellular interactions in vitro, co-culture systems have achieved tremendous success achieving a more realistic microenvironment of in vivo metabolism than monoculture system in the past several decades. Recently, the fields of tissue engineering and regenerative medicine have primarily focused on three-dimensional co-culture systems using cellular scaffolds, because of their physical and biological relevance to the extracellular matrix of actual tissues. This review discusses several materials and methods to create co-culture systems, including hydrogels, electrospun fibers, microfluidic devices, and patterning for biomimetic co-culture system and their applications for specific tissue regeneration. Consequently, we believe that culture systems with appropriate physical and biochemical properties should be developed, and direct or indirect cell-cell interactions in the remodeled tissue must be considered to obtain an optimal tissue-specific microenvironment.
大多数天然组织的细胞外基质包含多种类型的细胞，包括成纤维细胞、干细胞和内皮细胞，这些细胞直接或间接相互通讯以调节基质产生和细胞功能。为了在体外构建多细胞相互作用，在过去几十年中，共培养系统取得了巨大成功，与单培养系统相比，它实现了更接近体内代谢的真实微环境。最近，组织工程和再生医学领域主要关注使用细胞支架的三维共培养系统，因为它们与实际组织的细胞外基质具有物理和生物学相关性。本文综述了几种创建共培养系统的材料和方法，包括水凝胶、电纺纤维、微流控装置以及用于仿生共培养系统的图案化方法及其在特定组织再生中的应用。因此，我们认为应开发具有适当物理和生化特性的培养系统，并且必须考虑重塑组织中直接或间接的细胞间相互作用，以获得最佳的组织特异性微环境。
