Clermont University, Université d'Auvergne, IMTV, F-63000, Clermont-Ferrand, France.
BMC Complement Altern Med. 2013 Mar 1;13:51. doi: 10.1186/1472-6882-13-51.
Extracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root.
Hydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans.
Each extract presented significant α-glucosidase inhibition and antioxidant activities. Polygonum senegalensis leaf extracts were the most active in each in vitro assay with an IC50 = 1.5 μg/ml for α-glucosidase inhibition and an IC50 = 6.8 μg/ml for DPPH scavenging, - 4.5 μmol Fe II/g of dry matter - 9366 μmol Trolox / g DW - for FRAP and ORAC values, respectively. IC50 = 2.3 μg GA / ml for DCFH-DA assay. Concerning its antibacterial activity, a growth inhibitory effect was observed only against three Gram negative bacilli: B. subtilis, E. faecalis, S. aureus and the yeast C. albicans at high concentration.
The results showed that the semi alcoholic extract of the two studied plants possess α-glucosidase inhibitory activity, antioxidant potency, and low antibacterial effect.
在贝宁农村，Polygonum senegalensis（Polygonaceae）和Pseudocedrela kotschyi（Meliaceae）的提取物是两种重要的传统药用植物，用于治疗许多疾病，特别是 2 型糖尿病。本研究旨在研究这些植物提取物（Polygonum senegalensis 叶和 Pseudocedrela kotschyi 根）的 α-葡萄糖苷酶抑制、抗氧化和抗菌活性。
对水醇（50%）提取物进行了植物化学分析，并测试了其对酿酒酵母α-葡萄糖苷酶的抑制能力。采用 DPPH、ORAC、FRAP 和 DCFH-DA（基于细胞）测定法评估抗氧化活性。最后，通过对四种革兰氏阳性球菌（枯草芽孢杆菌、艰难梭菌、粪肠球菌、金黄色葡萄球菌）、三种革兰氏阴性杆菌（大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌）和酵母白色念珠菌的 MIC 测定评估了抗菌活性。
每种提取物均表现出显著的 α-葡萄糖苷酶抑制和抗氧化活性。在每种体外测定中，Polygonum senegalensis 叶提取物最为活跃，对 α-葡萄糖苷酶的抑制 IC50 为 1.5μg/ml，对 DPPH 的清除 IC50 为 6.8μg/ml，FRAP 和 ORAC 值分别为-4.5μmol Fe II/g 干物质-9366μmol Trolox/g DW。对于 DCFH-DA 测定，IC50 为 2.3μgGA/ml。关于其抗菌活性，仅在高浓度下观察到对三种革兰氏阴性杆菌（B. subtilis、E. faecalis、S. aureus）和酵母 C. albicans 的生长抑制作用。
结果表明，两种研究植物的半醇提取物具有 α-葡萄糖苷酶抑制活性、抗氧化能力和低抗菌作用。
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