Tao Xiangxiang, Yan Yifeng, Lu Linming, Chen Bing
Department of Pathology, Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Department of Forensic Pathology, Wannan Medical College, Wuhu, Anhui 241001, P.R. China.
Oncol Lett. 2017 Oct;14(4):4923-4929. doi: 10.3892/ol.2017.6818. Epub 2017 Aug 24.
Despite increasing evidence of the involvement of histone deacetylase (HDAC)10 in cancer tumorigenesis, the potential role of HDAC10 in colon cancer remains unclear. Oncomine database analysis revealed that HDAC10 mRNA was significantly upregulated in colon cancer. In an independent cohort, consistent with mRNA expression levels, constitutively high HDAC10 expression was observed in the cytoplasm and nucleus compared with in adjacent normal tissues (cytoplasm, 93.12±12.98 vs. 31.65±26.50%; nucleus, 84.16±19.23 vs. 68.64±19.00%). Cytoplasmic HDAC expression correlated with gender (r=0.265; P<0.05), lymph node metastasis (N stage; r=0.256; P<0.05) and distant metastasis (M stage; r=0.331; P<0.05) in paracarcinoma tissues. Cytoplasmic HDAC10 expression in tumors was not associated with the four DNA mismatch repair genes examined, but was negatively correlated with mutL homolog 1 (MLH1) (r=-0.244; P<0.05), mutS homolog (MSH)2 (r=-0.410; P<0.01) and MSH6 (r=-0.240; P<0.05) in paracarcinoma tissues. Similarly, nuclear HDAC10 expression was negatively correlated with MLH1 expression (r=-0.288; P<0.05). The findings of the current study suggest that HDAC10 expression is associated with good prognosis in colon cancer tissues and poor prognosis in paracarcinoma tissues with a potential involvement in DNA mismatch repair.
尽管越来越多的证据表明组蛋白去乙酰化酶（HDAC）10参与癌症肿瘤发生，但HDAC10在结肠癌中的潜在作用仍不清楚。Oncomine数据库分析显示，HDAC10 mRNA在结肠癌中显著上调。在一个独立队列中，与mRNA表达水平一致，与相邻正常组织相比，在细胞质和细胞核中观察到HDAC10持续高表达（细胞质，93.12±12.98%对31.65±26.50%；细胞核，84.16±19.23%对68.64±19.00%）。癌旁组织中细胞质HDAC表达与性别（r=0.265；P<0.05）、淋巴结转移（N分期；r=0.256；P<0.05）和远处转移（M分期；r=0.331；P<0.05）相关。肿瘤中细胞质HDAC10表达与所检测的四个DNA错配修复基因无关，但与癌旁组织中的错配修复蛋白1（MLH1）（r=-0.244；P<0.05）、错配修复蛋白2（MSH2）（r=-0.410；P<0.01）和MSH6（r=-0.240；P<0.05）呈负相关。同样，细胞核HDAC10表达与MLH1表达呈负相关（r=-0.288；P<0.05）。本研究结果表明，HDAC10表达与结肠癌组织的良好预后以及癌旁组织的不良预后相关，且可能参与DNA错配修复。
