Ataei Kachooei Saeed, Ranjbar Mohammad Mehdi, Ataei Kachooei Saba
Department of Bacterial Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Department of Animal Virology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Vet Res Forum. 2017 Summer;8(3):179-184. Epub 2017 Sep 15.
Members of gram-negative bacteria family , include a large number of important economically human and veterinary pathogens. Organisms belonging to the family can colonize in mucosal surfaces of the respiratory, alimentary, genital tracts and cause diseases in various mammals, birds, and reptiles. Hemorrhagic septicemia is an acute disease of cattle and buffaloes in tropical countries caused by serotype B:2. In the present study, the possible bactericidal activity of immune calf sera in the presence and absence of complement system was investigated. The results showed that B:2 is highly resistant to positive serum, containing high levels of IgG and IgM obtained from calves after vaccination, and complement activity in normal fresh calf serum. This organism also grew rapidly in the normal fresh calf serum and the mixture of positive serum as well as normal fresh calf serum. As a control test an strain was subjected to the same experiment and found completely sensitive to the bactericidal activity of complement in calf and guinea pig fresh sera. Results were indicative of the presence of inhibitory mechanism(s) in B:2 against bactericidal activity of immune calf serum and complement system.
革兰氏阴性菌家族的成员包括许多对人类和兽医具有重要经济意义的病原体。属于该家族的生物体可在呼吸道、消化道、生殖道的粘膜表面定殖，并在各种哺乳动物、鸟类和爬行动物中引起疾病。出血性败血症是热带国家牛和水牛的一种急性疾病，由B:2血清型引起。在本研究中，研究了免疫小牛血清在有和没有补体系统存在时可能的杀菌活性。结果表明，B:2对阳性血清具有高度抗性，该阳性血清含有接种疫苗后从小牛获得的高水平IgG和IgM，以及正常新鲜小牛血清中的补体活性。该生物体在正常新鲜小牛血清以及阳性血清与正常新鲜小牛血清的混合物中也生长迅速。作为对照试验，对一个菌株进行了相同的实验，发现其对小牛和豚鼠新鲜血清中的补体杀菌活性完全敏感。结果表明B:2中存在针对免疫小牛血清和补体系统杀菌活性的抑制机制。
