Academic Medical Center, University of Amsterdam, Department of Biomedical Engineering and Physics,, The Netherlands.
Netherlands Cancer Institute, Department of Surgery, Amsterdam, The Netherlands.
J Biomed Opt. 2017 Oct;22(10):1-4. doi: 10.1117/1.JBO.22.10.100502.
To accurately determine sample optical properties using single fiber reflectance spectroscopy (SFR), an absolute calibration of the reflectance is required. We investigated two SFR calibration methods, using a calibrated mirror and using the Fresnel reflection at the fiber tip as a reference. We compared these to commonly used calibration methods, using either Intralipid-20% in combination with Monte Carlo simulations or Spectralon as a reference. The Fresnel reflection method demonstrated the best reproducibility and yielded the most reliable result. We therefore recommend the Fresnel reflection method for the measured absolute reflectance calibration of SFR.
为了使用单光纤反射光谱学（SFR）准确确定样本光学特性，需要对反射率进行绝对校准。我们研究了两种 SFR 校准方法，一种是使用校准后的镜子，另一种是使用光纤尖端的菲涅尔反射作为参考。我们将这些方法与常用的校准方法进行了比较，这些方法要么使用 20%的 Intralipid 与蒙特卡罗模拟相结合，要么使用 Spectralon 作为参考。菲涅尔反射法表现出最佳的可重复性，并产生了最可靠的结果。因此，我们建议使用菲涅尔反射法对 SFR 的测量绝对反射率进行校准。
