从玉米须中快速回收高含量植物甾醇。

Zhang Haiyan, Cao Xiaowan, Liu Yong, Shang Fude

College of Life Science, Henan University, Dongjing Street, Jinming District, Kaifeng, Henan province, 475004, People's Republic of China.

College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan, 475004, China.

Chem Cent J. 2017 Oct 18;11(1):108. doi: 10.1186/s13065-017-0277-1.

BACKGROUND

Phytosterols have important physiological and officinal function.

METHODS

An efficient ultrasonic assisted extraction, purification and crystallization procedure of phytosterols was established from corn silk for the first time.

RESULTS

The orthogonal test was applied to optimize the process parameters and a maximum phytosterols recovery as high as 10.5886 mg/g was achieved by ultrasonic treatment for 55 min with liquid-solid ratio of 12:1 at 35 °C, 220 w. The ultrasonic extraction temperature (T, °C) has the most significant effect on extraction yield of phytosterols. An orthogonal crystallization test was performed and the optimal conditions [crystallization temperature of 8 °C, time of 12 h and solid-liquid ratio of 1:1 (g/ml)] afforded maximum phytosterols purity of 92.76 ± 0.43%.

CONCLUSIONS

An efficient extraction and crystallization procedure was established.

背景

植物甾醇具有重要的生理和药用功能。

方法

首次建立了从玉米须中高效超声辅助提取、纯化及结晶植物甾醇的方法。

结果

采用正交试验优化工艺参数，在35℃、220W条件下，液固比12:1超声处理55min，植物甾醇最大回收率高达10.5886mg/g。超声提取温度（T，℃）对植物甾醇提取率影响最为显著。进行了正交结晶试验，最佳条件[结晶温度8℃、时间12h、固液比1:1（g/ml）]下植物甾醇最大纯度为92.76±0.43%。