Price J E, Daniels L M, Campbell D E, Giavazzi R
Department of Cell Biology, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Clin Exp Metastasis. 1989 Jan-Feb;7(1):55-68. doi: 10.1007/BF02057181.
The metastatic behavior of the HT-29 human colorectal carcinoma cell line was studied following injection into nude mice by different routes. After intrasplenic injection, experimental metastases formed in the livers of most mice. Variant lines were established in culture from the liver lesions and from tumors growing at the site of injection, the spleen. Cells of the HT-29 LMM line exhibited slightly enhanced ability to form liver metastases compared with cells of the non-selected parent line. When injected i.v., the HT-29 cells produced only a few small experimental metastases in the lungs, but in most of the mice macroscopic tumors were found in various lymph nodes and the interscapular fat. Analyses of the distribution of IdUrd-labeled cells did not reveal a preferential localization of the HT-29 cells in sites where metastases subsequently formed. This suggested that the growth of the human colon carcinoma cells in those sites might be the result of a stimulatory interaction between the tumor and host cells as opposed to growth in sites such as the lungs, where numerous cells arrested after i.v. injection but only a few, small metastases were seen 60 days later.
通过不同途径将HT - 29人结肠癌细胞系注射到裸鼠体内后，对其转移行为进行了研究。脾内注射后，大多数小鼠的肝脏形成了实验性转移灶。从肝脏病变以及注射部位脾脏处生长的肿瘤中培养建立了变异株系。与未筛选的亲代细胞系相比，HT - 29 LMM株系的细胞形成肝转移的能力略有增强。静脉注射时，HT - 29细胞在肺部仅产生少数小的实验性转移灶，但在大多数小鼠中，在各种淋巴结和肩胛间脂肪中发现了肉眼可见的肿瘤。对IdUrd标记细胞分布的分析未显示HT - 29细胞在随后形成转移灶的部位有优先定位。这表明人结肠癌细胞在这些部位的生长可能是肿瘤与宿主细胞之间刺激相互作用的结果，这与在肺部等部位的生长情况相反，静脉注射后肺部会滞留大量细胞，但60天后仅可见少数小转移灶。
