Roschanski Nicole, Guenther Sebastian, Vu Thi Thu Tra, Fischer Jennie, Semmler Torsten, Huehn Stephan, Alter Thomas, Roesler Uwe
Freie Universitaet Berlin, Institute for Animal Hygiene and Environmental Health, Berlin, Germany.
Freie Universitaet Berlin, Institute of Food Safety and Food Hygiene, Berlin, Germany.
Euro Surveill. 2017 Oct;22(43). doi: 10.2807/1560-7917.ES.2017.22.43.17-00032.
Carbapenems belong to the group of last resort antibiotics in human medicine. Therefore, the emergence of growing numbers of carbapenemase-producing bacteria in food-producing animals or the environment is worrying and an important concern for the public health sector. In the present study, a set of 45 Enterobacteriaceae isolated from German retail seafood (clams and shrimps), sampled in 2016, were investigated by real-time PCR for the presence of carbapenemase-producing bacteria. One (ST10), isolated from a Venus clam () harvested in the Mediterranean Sea (Italy), contained the carbapenemase gene as part of the variable region of a class I integron. Whole-genome sequencing indicated that the integron was embedded in a Tn3-like transposon that also contained the fluoroquinolone resistance gene S1. Additional resistance genes such as the extended-spectrum beta-lactamase and the AmpC gene were also present in this isolate. Except , all resistance genes were located on an IncY plasmid. These results confirm previous observations that carbapenemase-producing bacteria have reached the food chain and are of increasing concern for public health.
碳青霉烯类药物属于人类医学中最后一道防线的抗生素类别。因此，在食用动物或环境中出现越来越多产碳青霉烯酶的细菌令人担忧，也是公共卫生部门的一个重要关切点。在本研究中，对2016年从德国零售海鲜（蛤蜊和虾）中分离出的一组45株肠杆菌科细菌进行了实时PCR检测，以确定是否存在产碳青霉烯酶的细菌。其中一株（ST10）从地中海（意大利）收获的维纳斯蛤蜊中分离得到，其碳青霉烯酶基因作为I类整合子可变区的一部分存在。全基因组测序表明，该整合子嵌入在一个类似Tn3的转座子中，该转座子还包含氟喹诺酮抗性基因S1。该分离株中还存在其他抗性基因，如超广谱β-内酰胺酶和AmpC基因。除了 ，所有抗性基因都位于一个IncY质粒上。这些结果证实了先前的观察结果，即产碳青霉烯酶的细菌已进入食物链，并且对公共卫生的关注度日益增加。
