Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, Liaocheng, Shandong, China.
J Cell Biochem. 2018 Jul;119(7):5118-5125. doi: 10.1002/jcb.26468. Epub 2018 Apr 6.
MicroRNAs (miRNAs) are aberrantly expressed in several tumors and play important role in tumorigenesis. However, little is known about the role of miR-613 in laryngeal squamous cell carcinoma (LSCC). We determined the expression of miR-613 in a panel of 30 LSCC specimens. Compared with the adjacent normal samples, 20 cases of LSCC tissues exhibited decreased expression of miR-613. The average expression of miR-613 in LSCC tissues was lower than in normal samples. Moreover, we demonstrated that exogenous expression of miR-613 suppressed LSCC cell proliferation, invasion, and blocked G1/S phase transition. We identified that 3-phosphoinositide-dependent protein kinase-1 (PDK1) was a direct target gene of miR-613 in LSCC cell. Overexpression of miR-613 suppressed PDK1 expression in LSCC cell. Furthermore, we demonstrated that PDK1 was upregulated in LSCC tissues. MiR-613 expression was inversely correlated with the expression of PDK1 in LSCC tissues. Moreover, we showed that PDK1 was involved in the miR-613-mediated cancer suppression of LSCC cell. These results suggested that miR-613 played as a tumor suppressor gene in LSCC partly by inhibiting PDK1 expression.
微小 RNA（miRNAs）在多种肿瘤中表达异常，在肿瘤发生中发挥重要作用。然而，miR-613 在喉鳞状细胞癌（LSCC）中的作用知之甚少。我们在 30 个 LSCC 标本中确定了 miR-613 的表达。与相邻的正常样本相比，20 例 LSCC 组织表现出 miR-613 的表达降低。LSCC 组织中 miR-613 的平均表达低于正常样本。此外，我们证明外源性表达 miR-613 可抑制 LSCC 细胞增殖、侵袭，并阻断 G1/S 期转变。我们确定 3-磷酸肌醇依赖性蛋白激酶-1（PDK1）是 LSCC 细胞中 miR-613 的直接靶基因。miR-613 的过表达可抑制 LSCC 细胞中 PDK1 的表达。此外，我们证明 PDK1 在 LSCC 组织中上调。miR-613 的表达与 LSCC 组织中 PDK1 的表达呈负相关。此外，我们表明 PDK1 参与了 miR-613 介导的 LSCC 细胞的肿瘤抑制作用。这些结果表明，miR-613 通过抑制 PDK1 的表达，在 LSCC 中部分发挥肿瘤抑制基因的作用。
