Santos M Aparecida, Florencio-Silva R, Teixeira C P, Sasso G Rodrigues da Silva, Marinho D Souza, Simões R S, Simões M J, Carbonel A Ferraz
a Universidade Federal De São Paulo , São Paulo ;
b Morphology and Genetics , Universidade Federal De São Paulo , São Paulo ;
Climacteric. 2016;19(1):77-84. doi: 10.3109/13697137.2015.1094783. Epub 2015 Nov 25.
Soy isoflavones have been shown to be an alternative to hormone therapy at menopause, without causing side-effects such as breast cancer. However, the effects of early and late treatment with isoflavones on the mammary gland remain controversial.
To investigate the effects of early and late treatment with soy isoflavones on the mammary gland of ovariectomized rats.
Thirty 3-month-old rats were ovariectomized and divided equally into groups: Control, treated with vehicle solution; or with 150 mg/kg/body weight of isoflavones by gavage; or subcutaneously treated with 10 μg/kg/body weight with 17β-estradiol. Treatments started 3 days (early treatment) or 30 days (late treatment) after ovariectomy and lasted for 30 consecutive days. Thereafter, the animals were euthanized and the mammary glands were removed and processed for paraffin embedding. Sections were stained with hematoxylin and eosin for histomorphometry or subjected to immunohistochemical detection of Ki-67 and VEGF-A.
The ductal, lobular and total epithelial fractions were similar between controls and the early/late isoflavone groups, but they were significantly higher in the groups treated with estradiol. In both epithelial and stromal regions, the immunoreactivity of VEGF-A and the percentage of Ki-67-positive cells were significantly higher in the groups treated with estradiol, while they were similar in the early/late isoflavone groups and control groups.
Our results indicate that early and late treatment with soy isoflavones at the dose of 150 mg/kg/body weight does not show proliferative and angiogenic effects on the mammary gland of ovariectomized rats.
大豆异黄酮已被证明是更年期激素治疗的一种替代方法，不会引起如乳腺癌等副作用。然而，异黄酮早期和晚期治疗对乳腺的影响仍存在争议。
研究大豆异黄酮早期和晚期治疗对去卵巢大鼠乳腺的影响。
将30只3月龄大鼠去卵巢，并平均分为几组：对照组，给予赋形剂溶液；或通过灌胃给予150毫克/千克体重的异黄酮；或皮下给予10微克/千克体重的17β-雌二醇。治疗在去卵巢后3天（早期治疗）或30天（晚期治疗）开始，持续30天。此后，对动物实施安乐死，取出乳腺并进行石蜡包埋处理。切片用苏木精和伊红染色用于组织形态计量学分析，或进行Ki-67和VEGF-A的免疫组化检测。
对照组与异黄酮早期/晚期治疗组之间的导管、小叶和总上皮部分相似，但在雌二醇治疗组中显著更高。在上皮和基质区域，VEGF-A的免疫反应性和Ki-67阳性细胞百分比在雌二醇治疗组中显著更高，而异黄酮早期/晚期治疗组与对照组相似。
我们的结果表明，以150毫克/千克体重的剂量对去卵巢大鼠进行大豆异黄酮早期和晚期治疗，对其乳腺没有增殖和血管生成作用。
