González Sonia, Gutie Rrez-Díaz Isabel, Lo Pez Patricia, Suárez Ana, Fernández-Navarro Tania, Sánchez Borja, Margolles Abelardo
Universidad de Oviedo.
Nutr Hosp. 2017 Jul 28;34(4):934-941. doi: 10.20960/nh.546.
Systemic lupus erythematosus (SLE) is a chronic infl ammatory disease of autoimmune nature, in which oxidative stress is implicated.
Compare the concentrations of dietary and blood antioxidants, as well as gut microbiota, with serum malondialdehyde (MDA) and C reactive protein (CRP) in 21 subjects suffering from non-active systemic lupus erythematosus (SLE) and 21 age and gender-matched controls.
General biochemical parameters and CRP were determined by enzymatic methods: copper, zinc and selenium by inductively coupled plasma mass spectrometry (ICP-MS), MDA and total antioxidant capacity (TAC) by spectrophotometric methods, gut microbiota by metagenomic analyses and dietary intake by means of food frequency questionnaire.
No significant differences were found in diet between lupus patients and the control group, with the exception of trans fatty acids intake, which was higher in patients. In addition, higher concentration of serum copper and lower of zinc in SLE were found. Serum copper was positively associated with CRP and also, this protein with the proportion of Lentisphaerae, ProteobacteriaandVerrucomicrobiain feces. Moreover, MDA levels displayed inverse correlations with the Cyanobacteriaand Firmicutesgroups, while Actinobacteria showed a positive association. The lupus subjects with presence of anti-SSA/Ro were related to higher levels of serum zinc.
These results could be useful in the future to go deeper into the understanding of this complex disease.
系统性红斑狼疮（SLE）是一种自身免疫性慢性炎症性疾病，其中涉及氧化应激。
比较21例非活动性系统性红斑狼疮（SLE）患者和21例年龄及性别匹配的对照者的膳食和血液抗氧化剂浓度、肠道微生物群与血清丙二醛（MDA）和C反应蛋白（CRP）水平。
采用酶法测定一般生化参数和CRP；采用电感耦合等离子体质谱法（ICP-MS）测定铜、锌和硒；采用分光光度法测定MDA和总抗氧化能力（TAC）；采用宏基因组分析测定肠道微生物群；通过食物频率问卷评估膳食摄入量。
狼疮患者与对照组在饮食方面未发现显著差异，但患者的反式脂肪酸摄入量较高。此外，发现SLE患者血清铜浓度较高而锌浓度较低。血清铜与CRP呈正相关，并且该蛋白与粪便中 Lentisphaerae、变形菌门和疣微菌门的比例相关。此外，MDA水平与蓝细菌和厚壁菌门呈负相关，而放线菌门呈正相关。存在抗SSA/Ro的狼疮患者血清锌水平较高。
这些结果未来可能有助于更深入地了解这种复杂疾病。
