Adamski Patryk, Byczkowska-Rostkowska Zuzanna, Gajewska Joanna, Zakrzewski Arkadiusz Józef, Kłębukowska Lucyna
Department of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, Poland.
Microorganisms. 2023 Apr 19;11(4):1065. doi: 10.3390/microorganisms11041065.
Milk, due to its diversity in terms of its nutritional content, is an important element of the human diet, as well as a good medium for the development of bacteria. The genus contains ubiquitous aerobic, rod-shaped, endospore-producing gram-positive bacteria. Representatives of the group and the group contribute to shortening the shelf life of milk and dairy products by degrading milk components and its additives. They also produce a number of heat-stable toxins and can cause a number of ailments, mainly in the digestive system. The aim of this research was to identify sp. strains isolated from raw milk and to determine their antibiotic resistance. Strains isolated from raw milk samples ( = 45) were identified by MALDI-TOF MS. Ninety strains of sp. were identified, for which the antibiotic resistance phenotype was determined. A total of 90 strains of were classified in five groups (the group ( = 35), ( = 7), the group ( = 29), ( = 16), and sp. ( = 3). All isolates were susceptible to chloramphenicol and meropenem. The antibiotic resistance profiles of the tested groups of spp. differed from each other, which is of particular concern in relation to multidrug-resistant representatives of the group resistant to cefotaxime (94.29%), ampicillin (88.57%), rifampicin (80%), and norfloxacin (65.71%). Our study provides data on the prevalence and antibiotic sensitivity of sp. In raw milk, suggesting a potential risk to health and the dairy industry.
牛奶因其营养成分的多样性,是人类饮食的重要组成部分,也是细菌生长的良好培养基。该属包含无处不在的需氧、杆状、产芽孢革兰氏阳性细菌。该组和该组的代表通过降解牛奶成分及其添加剂,导致牛奶和乳制品的保质期缩短。它们还会产生多种热稳定毒素,并能引发多种疾病,主要是消化系统疾病。本研究的目的是鉴定从生牛奶中分离出的 种菌株,并确定它们的抗生素抗性。通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对从生牛奶样品(n = 45)中分离出的菌株进行鉴定。鉴定出 90 株 种菌株,并确定了它们的抗生素抗性表型。总共 90 株 被分为五组(该组(n = 35)、 (n = 7)、该组(n = 29)、 (n = 16)和 种(n = 3)。所有分离株对氯霉素和美罗培南敏感。所测试的 种菌株组的抗生素抗性谱彼此不同,这对于耐头孢噻肟(94.29%)、氨苄青霉素(88.57%)、利福平(80%)和诺氟沙星(65.71%)的该组多重耐药代表菌株尤其令人担忧。我们的研究提供了关于生牛奶中 种菌株流行情况和抗生素敏感性的数据,表明对健康和乳制品行业存在潜在风险。