Clinical Instructor, Periodontology and Oral Implantology, School of Dental Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Associate Professor, Periodontology and Oral Implantology, School of Dental Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Implant Dent. 2017 Dec;26(6):899-903. doi: 10.1097/ID.0000000000000697.
To use a modified direct contact test (DCT) to evaluate the bacterial growth on 3 commercially available implants.
Streptococcus salivarius growth was tested on 1 machined surface and 2 commercial rough surface implants. Seven implants from each group were fixed to the cover of a 96-well microtiter plate. Bacterial suspension was placed on each implant, and the plate was incubated at 37°C for 1 hour to allow the suspension fluid to evaporate. Fresh medium was added to 7 corresponding wells in the microtiter plate, and the cover holding the implants was placed on the plate to enable immersion of the implant. Noncontaminated implant samples served as control. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 hours.
There was a significant S. salivarius growth in all groups of test implants compared with the control. There was no significant difference between bacterial growth kinetics on treated and nontreated implant surfaces (P = 0.241).
Using the DCT, we found no difference in bacterial growth between machined and rough surface implants.
使用改良的直接接触试验（DCT）评估 3 种市售植入物上的细菌生长情况。
在 1 个机械加工表面和 2 种商业粗糙表面植入物上测试唾液链球菌的生长情况。每组 7 个植入物固定在 96 孔微量滴定板的盖子上。将细菌悬液置于每个植入物上，将平板在 37°C 下孵育 1 小时，以使悬浮液蒸发。将新鲜培养基添加到微量滴定板的 7 个相应孔中，并将带有植入物的盖子放置在平板上，以使植入物浸没。未污染的植入物样本作为对照。使用分光光度法在 650nm 处监测 24 小时的细菌生长情况。
与对照相比，所有测试植入物组的 S. salivarius 生长均有显著增加。处理和未处理植入物表面的细菌生长动力学之间无显著差异（P=0.241）。
使用 DCT，我们发现机械加工和粗糙表面植入物之间的细菌生长没有差异。
