Wertz P W, Madison K C, Downing D T
Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242.
J Invest Dermatol. 1989 Jan;92(1):109-11. doi: 10.1111/1523-1747.ep13071317.
In the present study, we demonstrate that human stratum corneum contains covalently bound lipids accounting for 1.4% of the dry weight of the tissue. The major component (53.3% of the total by weight) is a ceramide (CER-A) consisting of 30 through 34-carbon omega-hydroxyacids amide-linked to sphingosine. The other bound lipids in human stratum corneum include fatty acids (12.7%), omega-hydroxy acids (9.4%), and a second, more polar, omega-hydroxyacid-containing ceramide (CER-B, 24.8%). The predominant omega-hydroxyacids in both ceramides, as well as the free hydroxyacid fraction, are the 30-carbon saturated and 32- and 34-carbon monoenoic species. The bound fatty acids consist largely of 14 through 22-carbon saturated species, but significant proportions of monoenoic species and linoleic acid are also present. Psoriatic scale contains a similar total concentration of the same covalently bound lipids, but the proportions of the individual bound lipids are different from those found in normal stratum corneum. It is suggested that the principal function of the covalently bound lipids in human stratum corneum is the formation of a lipid envelope on the outer surface of the keratinized cells.
在本研究中，我们证明人体角质层含有共价结合脂质，其占该组织干重的1.4%。主要成分（占总重量的53.3%）是一种神经酰胺（CER - A），它由30至34个碳原子的ω - 羟基酸与鞘氨醇酰胺连接而成。人体角质层中的其他结合脂质包括脂肪酸（12.7%）、ω - 羟基酸（9.4%）以及第二种极性更强的含ω - 羟基酸的神经酰胺（CER - B，24.8%）。两种神经酰胺中的主要ω - 羟基酸以及游离羟基酸部分，均为30个碳原子的饱和酸以及32和34个碳原子的单烯酸。结合脂肪酸主要由14至22个碳原子的饱和酸组成，但单烯酸和亚油酸也占相当比例。银屑病鳞屑中相同共价结合脂质的总浓度与之相似，但各结合脂质的比例与正常角质层中的不同。有人认为，人体角质层中共价结合脂质的主要功能是在角质化细胞外表面形成脂质包膜。
