Wertz P W, Downing D T
Biochim Biophys Acta. 1987 Jan 13;917(1):108-11. doi: 10.1016/0005-2760(87)90290-6.
Pig epidermis was heat separated, and the stratum corneum was isolated after trypsinization. Exhaustive extraction of the stratum corneum fraction with chloroform/methanol mixtures yielded 14.7% lipid on a dry weight basis. After mild saponification of the extracted residue, additional lipid could be extracted which accounted for 2.1% of the stratum corneum weight. This bound lipid proved to consist mainly (91.9%) of N-(omega-hydroxyacyl)sphingosines in which the amide-linked omega-hydroxyacids were 28 to 34 carbon atoms in length. The release of this lipid by mild alkaline hydrolysis indicates that it is bound through an ester linkage. Half of the hydroxyceramide molecules reacted in situ with acidic acetone, suggesting that half of these molecules are attached to the stratum corneum through the omega-hydroxyl function, while the other half may be linked through one of the hydroxyl groups of the sphingosine.
猪表皮经热分离，胰蛋白酶消化后分离出角质层。用氯仿/甲醇混合物彻底萃取角质层部分，以干重计得到14.7%的脂质。对提取的残渣进行温和皂化后，可提取出额外的脂质，其占角质层重量的2.1%。这种结合脂质主要（91.9%）由N-（ω-羟基酰基）鞘氨醇组成，其中酰胺连接的ω-羟基酸长度为28至34个碳原子。通过温和碱性水解释放出这种脂质，表明它是通过酯键结合的。一半的羟基神经酰胺分子与酸性丙酮原位反应，表明这些分子的一半通过ω-羟基官能团附着在角质层上，而另一半可能通过鞘氨醇的羟基之一连接。
