Gao Bei, Chi Liang, Tu Pengcheng, Bian Xiaoming, Thomas Jesse, Ru Hongyu, Lu Kun
NIH West Coast Metabolomics Center, University of California, Davis, CA 95616, United States; Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
Toxicol Lett. 2018 Feb;283:52-57. doi: 10.1016/j.toxlet.2017.10.023. Epub 2017 Oct 31.
The gut microbiome has tremendous potential to impact health and disease. Various environmental toxicants, including insecticides, have been shown to alter gut microbiome community structures. However, the mechanism that compositionally and functionally regulates gut microbiota remains unclear. Quorum sensing is known to modulate intra- and interspecies gene expression and coordinate population responses. It is unknown whether quorum sensing is disrupted when environmental toxicants cause perturbations in the gut microbiome community structure. To reveal the response of the quorum-sensing system to environmental exposure, we use a combination of Illumina-based 16S rRNA gene amplicon and shotgun metagenome sequencing to examine the impacts of a widely used organophosphate insecticide, malathion, on the gut microbiome trajectory, quorum sensing system and behaviors related to quorum sensing, such as motility and pathogenicity. Our results demonstrated that malathion perturbed the gut microbiome development, quorum sensing and quorum sensing related behaviors. These findings may provide a novel mechanistic understanding of the role of quorum-sensing in the gut microbiome toxicity of malathion.
肠道微生物群对健康和疾病具有巨大的影响潜力。包括杀虫剂在内的各种环境毒物已被证明会改变肠道微生物群的群落结构。然而，在组成和功能上调节肠道微生物群的机制仍不清楚。群体感应已知可调节种内和种间基因表达并协调群体反应。当环境毒物导致肠道微生物群落结构紊乱时，群体感应是否会受到干扰尚不清楚。为了揭示群体感应系统对环境暴露的反应，我们结合基于Illumina的16S rRNA基因扩增子和鸟枪法宏基因组测序，来研究一种广泛使用的有机磷杀虫剂马拉硫磷对肠道微生物群轨迹、群体感应系统以及与群体感应相关行为（如运动性和致病性）的影响。我们的结果表明，马拉硫磷扰乱了肠道微生物群的发育、群体感应以及与群体感应相关的行为。这些发现可能为群体感应在马拉硫磷肠道微生物毒性中的作用提供一种新的机制理解。
