Department of Gastroenterology, the PLA No. 59 Hospital, Kaiyuan 661600, Yunnan Province, China.
Department of Gastroenterology, The Second Affiliated Hospital, Kunming Medical University, Kunming 650101, Yunnan Province, China.
World J Gastroenterol. 2017 Oct 14;23(38):7009-7015. doi: 10.3748/wjg.v23.i38.7009.
To investigate the expression of annexin II in gastric carcinoma and its role in the metastasis of gastric cancer.
The expression of annexin II in 51 cases of gastric carcinoma and 24 cases of adjacent tissues was detected by immunohistochemistry. The relationship between annexin II and clinical features of gastric cancer was analyzed. Annexin II specific siRNA was used to inhibit the expression of annexin II in gastric cancer HGC-27 cells, and the effects of annexin II on the migration and secretion of matrix metalloproteinases (MMPs) were observed.
The positive rate of annexin II protein was 82.4% in gastric cancer tissues and 37.5% in adjacent tissues. There was significant difference between the two groups ( < 0.01); and the positive expression of annexin II was not related to the sex and age of the patients ( > 0.05). The expression of annexin II protein was correlated with tumor size, histological differentiation, TNM stage, Lymph node metastasis and other clinical features were significantly correlated, the difference was statistically significant ( < 0.05). Inhibition of annexin II expression, gastric cancer HGC-27 cells migration and secretion of MMPs were significantly decreased, the difference was statistically significant ( < 0.05).
Annexin II is highly expressed in gastric cancer tissues, annexin II protein expression is related to tumor size, histological differentiation, TNM staging, lymph node metastasis and other clinical features were significantly correlated. Annexin II high expression can promote the invasion and metastasis of gastric cancer.
探讨膜联蛋白Ⅱ在胃癌中的表达及其在胃癌转移中的作用。
采用免疫组化法检测 51 例胃癌组织和 24 例癌旁组织中膜联蛋白Ⅱ的表达，分析膜联蛋白Ⅱ与胃癌临床特征的关系。用 Annexin II 特异性 siRNA 抑制胃癌 HGC-27 细胞中 Annexin II 的表达，观察 Annexin II 对基质金属蛋白酶（MMPs）迁移和分泌的影响。
胃癌组织中膜联蛋白Ⅱ蛋白的阳性率为 82.4%，癌旁组织中为 37.5%，两组比较差异有统计学意义（ < 0.01）；且膜联蛋白Ⅱ的阳性表达与患者的性别、年龄无关（ > 0.05）。膜联蛋白Ⅱ蛋白的表达与肿瘤大小、组织学分化、TNM 分期、淋巴结转移等临床特征密切相关，差异有统计学意义（ < 0.05）。抑制 Annexin II 的表达后，胃癌 HGC-27 细胞的迁移和 MMPs 的分泌明显减少，差异有统计学意义（ < 0.05）。
膜联蛋白Ⅱ在胃癌组织中高表达， Annexin II 蛋白的表达与肿瘤大小、组织学分化、TNM 分期、淋巴结转移等临床特征密切相关。 Annexin II 高表达可促进胃癌的侵袭和转移。
