Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL 61604, USA.
Bioresour Technol. 2012 Jan;104:410-6. doi: 10.1016/j.biortech.2011.10.099. Epub 2011 Nov 10.
This study reports a new yeast strain of Clavispora NRRL Y-50464 that is able to utilize cellobiose as sole source of carbon and produce sufficient native β-glucosidase enzyme activity for cellulosic ethanol production using SSF. In addition, this yeast is tolerant to the major inhibitors derived from lignocellulosic biomass pre-treatment such as 2-furaldehyde (furfural) and 5-(hydroxymethyl)-2-furaldehyde (HMF), and converted furfural into furan methanol in less than 12h and HMF into furan-2,5-dimethanol within 24h in the presence of 15 mM each of furfural and HMF. Using xylose-extracted corncob residue as cellulosic feedstock, an ethanol production of 23 g/l was obtained using 25% solids loading at 37 °C by SSF without addition of exogenous β-glucosidase. Development of this yeast aids renewable biofuels development efforts for economic consolidated SSF bio-processing.
本研究报道了一株新的酵母菌株 Clavispora NRRL Y-50464，它能够利用纤维二糖作为唯一的碳源，并在固态发酵（SSF）中产生足够的天然β-葡萄糖苷酶活性，用于生产纤维素乙醇。此外，该酵母能够耐受木质纤维素生物质预处理产生的主要抑制剂，如 2-糠醛（糠醛）和 5-羟甲基-2-糠醛（HMF），并在 15mM 糠醛和 HMF 存在下，在 12 小时内将糠醛转化为呋喃甲醇，在 24 小时内将 HMF 转化为呋喃-2,5-二甲醇。使用提取木糖后的玉米芯残渣作为纤维素原料，在 37°C 下，采用 25%的固含量进行 SSF，无需添加外源β-葡萄糖苷酶，可获得 23g/L 的乙醇产量。该酵母的开发有助于可再生生物燃料的发展，为经济一体化的 SSF 生物加工提供支持。
