University of Sfax-Tunisia, FSS, Department of Life Sciences, Laboratory of Biodiversity and Aquatic Ecosystems Ecology and Planktonology, Street of Soukra Km 3.5, BP 1171, CP 3000 Sfax, Tunisia.
University of Sfax-Tunisia, FSS, Department of Life Sciences, Laboratory of Biodiversity and Aquatic Ecosystems Ecology and Planktonology, Street of Soukra Km 3.5, BP 1171, CP 3000 Sfax, Tunisia.
Int J Biol Macromol. 2018 Jul 1;113:813-820. doi: 10.1016/j.ijbiomac.2018.02.152. Epub 2018 Feb 27.
The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25-150μg·mL) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P<0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.
研究了从蓝藻拟柱胞藻 NCC466（CFv-PS）中提取的粗多糖的体外抗氧化、细胞毒性和细胞保护特性以及体内肝保护活性。CFv-PS 被鉴定为杂多糖，分子量为 63.79kDa，在体外试验中表现出较强的抗氧化活性，呈浓度依赖性。此外，在测试浓度范围内（25-150μg·mL），CFv-PS 对 HepG2 人肝癌细胞没有诱导细胞毒性作用，同时能防止它们受到 Cd 的影响。此外，在 Cd 诱导的大鼠肝毒性中，CFv-PS 预处理显著（P<0.05）降低了丙氨酸氨基转移酶、天冬氨酸氨基转移酶、胆红素、MDA、蛋白质羰基和 DNA 损伤的水平，并显著增加了肝组织中的酶活性。这些发现表明，蓝藻拟柱胞藻是具有抗氧化、细胞保护和肝保护特性的天然产物的潜在来源。
